Surface Expression of Kynurenine 3-Monooxygenase Promotes Proliferation and Metastasis in Triple-Negative Breast Cancers

doi:10.1177/10732748211009245

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/24051

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/24051

Title:	Surface Expression of Kynurenine 3-Monooxygenase Promotes Proliferation and Metastasis in Triple-Negative Breast Cancers
Authors:	Lai, MH;Liao, CH;Tsai, NM;Chang, KF;Liu, CC;Chiu, YH;Huang, KC;Lin, CS
Keywords:	protein dislocation;cell membrane;kynurenine pathway;tumor biomarker;tumorigenesis
Date:	2021
Issue Date:	2022-08-05T09:47:02Z (UTC)
Publisher:	SAGE PUBLICATIONS INC
ISSN:	1073-2748
Abstract:	Kynurenine 3-monooxygenase (KMO) is the pivotal enzyme in the kynurenine pathway and is located on the mitochondrial outer membrane. The dysregulation of KMO leads to various neurodegenerative diseases; however, it is rarely mentioned in cancer progression. Our previous study showed that KMO overexpression in canine mammary gland tumors (cMGT) is associated with poor prognosis in cMGT patients. Surprisingly, it was also found that KMO can be located on the cell membranes of cMGT cells, unlike its location in normal cells, where KMO is expressed only within the cytosol. Since cMGT and human breast cancer share similar morphologies and pathogenesis, this study investigated the possibility of detecting surface KMO in human breast cancers and the role of surface KMO in tumorigenesis. Using immunohistochemistry (IHC), flow cytometry (FC), immunofluorescence assay (IFA), and transmission electron microscopy (TEM), we demonstrated that KMO can be aberrantly and highly expressed on the cell membranes of breast cancer tissues and in an array of cell lines. Masking surface KMO with anti-KMO antibody reduced the cell viability and inhibited the migration and invasion of the triple-negative breast cancer cell line, MDA-MB-231. These results indicated that aberrant surface expression of KMO may be a potential therapeutic target for human breast cancers.
URI:	http://dx.doi.org/10.1177/10732748211009245 https://www.webofscience.com/wos/woscc/full-record/WOS:000755668400001 https://ir.csmu.edu.tw:8080/handle/310902500/24051
Relation:	CANCER CONTROL ,2021,v28
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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