Optical profile: A key determinant of antibacterial efficacy of photodynamic therapy in dentistry

doi:10.1016/j.pdpdt.2021.102461

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/24030

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/24030

Title:	Optical profile: A key determinant of antibacterial efficacy of photodynamic therapy in dentistry
Authors:	Su, CT;Chen, CJ;Chen, CM;Chen, CC;Ma, SH;Wu, JH
Keywords:	Photodynamic therapy;Periodontal disease;Methylene blue;Antimicrobial efficacy;Optical profile
Date:	2021
Issue Date:	2022-08-05T09:46:44Z (UTC)
Publisher:	ELSEVIER
ISSN:	1572-1000
Abstract:	Background: Periodontal disease effects 20-50% of the population worldwide, posing a global health challenge. It has been reported to be more prevalent among adults. Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) is an important organism associated with localized juvenile periodontitis. Photodynamic therapy (PDT) has been widely utilized for the treatment of periodontal disease; however, the effect of laser (light) profile on the antibacterial efficacy of PDT remains to be established. The quantitative measurement of laser profile is required to confirm the in vitro efficacy of PDT. Aim: In the present study, a low cost PDT system comprising of six copper tube waveguides (CTW) was developed to provide more uniform irradiation of the culture plate. Methods: The antibacterial effect of PDT, in combination with 200 mu g/mL methylene blue (MB) as photosensitizer and 60 sec of irradiation, was studied on A. actinomycetemcomitans and Streptococcus mutans (S. mutans). In the present case, 660 nm laser guided with unpolished CTW, polished CTW, and optical fiber waveguide (OFW) provided radiant exposure of 0.86, 1.38, and 1.36 J/cm2, respectively, for a 24-well culture plate. Results: The designed PDT system provided antimicrobial efficacy of 98% and 91% for A. actinomycetemcomitans and S. mutans, respectively, which was significantly higher as compared to OFW guided PDT. Conclusion: The results of the study highlighted the importance of laser profile as a key parameter that determines the survival rate of bacteria at the edge of the culture plate. Thus, the dose of PDT at the margin of optical profile is important for antibacterial activity for in vitro evaluation.
URI:	http://dx.doi.org/10.1016/j.pdpdt.2021.102461 https://www.webofscience.com/wos/woscc/full-record/WOS:000696990900003 https://ir.csmu.edu.tw:8080/handle/310902500/24030
Relation:	PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY ,2021,v35
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	177	View/Open

Loading...