C-C Motif Chemokine Ligand-17 as a Novel Biomarker and Regulator of Epithelial Mesenchymal Transition in Renal Fibrogenesis

doi:10.3390/cells10123345

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/23788

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/23788

Title:	C-C Motif Chemokine Ligand-17 as a Novel Biomarker and Regulator of Epithelial Mesenchymal Transition in Renal Fibrogenesis
Authors:	Hsieh, YH;Wang, WC;Hung, TW;Lee, CC;Tsai, JP
Keywords:	chronic kidney disease;epithelial mesenchymal transition;CCL17;unilateral urethral obstruction
Date:	2021
Issue Date:	2022-08-05T09:42:54Z (UTC)
Publisher:	MDPI
Abstract:	CCL17, a chemotactic cytokine produced by macrophages, is known to promote inflammatory and fibrotic effects in multiple organs, but its role in mediating renal fibrosis is unclear. In our study cohort of 234 chronic kidney disease (CKD) patients and 65 healthy controls, human cytokine array analysis revealed elevated CCL17 expression in CKD that correlated negatively with renal function. The area under the receiver operating characteristic curve of CCL17 to predict the development of CKD stages 3b-5 was 0.644 (p < 0.001), with the optimal cut-off value of 415.3 ng/mL. In vitro over-expression of CCL17 in HK2 cells had no effect on cell viability, but increased cell motility and the expression of alpha-SMA, vimentin and collagen I, as shown by western blot analysis. In a unilateral ureteral obstruction (UUO) mouse model, we observed significantly increased interstitial fibrosis and renal tubule dilatation by Masson's Trichrome and H&E staining, and markedly increased expression of CCL17, vimentin, collagen I, and alpha-SMA by IHC stain, qRTPCR, and western blotting. CCL17 induced renal fibrosis by promoting the epithelial-mesenchymal transition, resulting in ECM accumulation. CCL17 may be a useful biomarker for predicting the development of advanced CKD.
URI:	http://dx.doi.org/10.3390/cells10123345 https://www.webofscience.com/wos/woscc/full-record/WOS:000743053600001 https://ir.csmu.edu.tw:8080/handle/310902500/23788
Relation:	CELLS ,2021,v10,issue 12
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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