The Protective Effects of alpha-Mangostin Attenuate Sodium Iodate-Induced Cytotoxicity and Oxidative Injury via Mediating SIRT-3 Inactivation via the PI3K/AKT/PGC-1 alpha Pathway

doi:10.3390/antiox10121870

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/23785

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/23785

Title:	The Protective Effects of alpha-Mangostin Attenuate Sodium Iodate-Induced Cytotoxicity and Oxidative Injury via Mediating SIRT-3 Inactivation via the PI3K/AKT/PGC-1 alpha Pathway
Authors:	Chuang, CJ;Wang, ML;Yeh, JH;Chen, TC;Tsou, SC;Lee, YJ;Chang, YY;Lin, HW
Keywords:	age-related macular degeneration (AMD);oxidative stress;retinal pigment epithelial (RPE);alpha-mangostin (alpha-MG);antiapoptotic;antioxidant
Date:	2021
Issue Date:	2022-08-05T09:42:50Z (UTC)
Publisher:	MDPI
Abstract:	It is well known that age-related macular degeneration (AMD) is an irreversible neurodegenerative disease that can cause blindness in the elderly. Oxidative stress-induced retinal pigment epithelial (RPE) cell damage is a part of the pathogenesis of AMD. In this study, we evaluated the protective effect and mechanisms of alpha-mangostin (alpha-mangostin, alpha-MG) against NaIO3-induced reactive oxygen species (ROS)-dependent toxicity, which activates apoptosis in vivo and in vitro. MTT assay and flow cytometry demonstrated that the pretreatment of ARPE-19 cells with alpha-MG (0, 3.75, 7.5, and 15 mu M) significantly increased cell viability and reduced apoptosis from NaIO3-induced oxidative stress in a concentration-dependent manner, which was achieved by the inhibition of Bax, cleaved PARP-1, cleaved caspase-3 protein expression, and enhancement of Bcl-2 protein. Furthermore, pre-incubation of ARPE-19 cells with alpha-MG markedly inhibited the intracellular ROS and extracellular H2O2 generation via blocking of the abnormal enzyme activities of superoxide dismutase (SOD), the downregulated levels of catalase (CAT), and the endogenous antioxidant, glutathione (GSH), which were regulated by decreasing PI3K-AKT-PGC-1 alpha-STRT-3 signaling in ARPE-19 cells. In addition, our in vivo results indicated that alpha-MG improved retinal deformation and increased the thickness of both the outer nuclear layer and inner nuclear layer by inhibiting the expression of cleaved caspase-3 protein. Taken together, our results suggest that alpha-MG effectively protects human ARPE-19 cells from NaIO3-induced oxidative damage via antiapoptotic and antioxidant effects.
URI:	http://dx.doi.org/10.3390/antiox10121870 https://www.webofscience.com/wos/woscc/full-record/WOS:000735480600001 https://ir.csmu.edu.tw:8080/handle/310902500/23785
Relation:	ANTIOXIDANTS ,2021,v10,issue 12
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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