Cetyltrimethylammonium Bromide Disrupts Mesenchymal Characteristics of Human Tongue Squamous Cell Carcinoma SCC4 Cells Through Modulating Canonical TGF-beta/Smad/miR-181b/TIMP3 Signaling Pathway

doi:10.21873/anticanres.15429

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/23783

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/23783

Title:	Cetyltrimethylammonium Bromide Disrupts Mesenchymal Characteristics of Human Tongue Squamous Cell Carcinoma SCC4 Cells Through Modulating Canonical TGF-beta/Smad/miR-181b/TIMP3 Signaling Pathway
Authors:	Yue, CH;Chen, CH;Pan, YR;Chen, YP;Huang, FM;Lee, CJ
Keywords:	Cetyltrimethylammonium bromide;CTAB;tongue squamous cell carcinoma;TSCC;epithelial-to-mesenchymal transition;EMT;transforming growth factor-βTGF-&beta
Date:	2021
Issue Date:	2022-08-05T09:42:49Z (UTC)
Publisher:	INT INST ANTICANCER RESEARCH
ISSN:	0250-7005
Abstract:	Background/Aim: This study investigated the anti metastatic effects of cetyltrimethylammonium bromide (CTAB) on tongue squamous cell carcinoma (TSCC) SCC4 cells. Materials and Methods: Cell morphology, viability, cell cycle distribution, adhesion, migration, invasion and the expression levels of associated proteins were examined using microscopy, WST-1, wound-healing, Boyden chamber assays, and western blotting, respectively. Results: CTAB significantly affected SCC4 cell morphology from spindle-shaped to cobblestone-shaped and resulted in loss of adherence. CTAB significantly inhibited cell adhesion, migration, and invasion of SCC4 cells, independent of cell viability. CTAB reduced expression of matrix metalloproteinases (MMPs) such as MMP3, MMP7, and MMP14 in a concentration-dependent manner, while it increased expression of tissue inhibitors of metalloproteinase 3 (TIMP3). In addition, CTAB reduced the phosphorylation of mothers against decapentaplegic homolog 2/3 (Smad2/3) proteins, which mediated CTAB-inhibited migration and invasion in SCC4 cells. These effects were reversed by TGF-beta 1. Conclusion: CTAB attenuates the mesenchymal characteristics through upregulation of TIMP3 by inhibiting the canonical TGF-beta/Smad/miR-181b/TIMP3 signaling involved in extracellular matrix remodeling in SCC4 cells and might be a promising anti-metastatic therapeutic agent for TSCC.
URI:	http://dx.doi.org/10.21873/anticanres.15429 https://www.webofscience.com/wos/woscc/full-record/WOS:000725492100024 https://ir.csmu.edu.tw:8080/handle/310902500/23783
Relation:	ANTICANCER RESEARCH ,2021,v41,issue 12, P6095-6104
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	155	View/Open

Loading...