Comparison of Two Quantitative PCR-Based Assays for Detection of Minimal Residual Disease in B-Precursor Acute Lymphoblastic Leukemia Harboring Three Major Fusion Transcripts

doi:10.1016/j.jmoldx.2021.07.008

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/23716

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/23716

Title:	Comparison of Two Quantitative PCR-Based Assays for Detection of Minimal Residual Disease in B-Precursor Acute Lymphoblastic Leukemia Harboring Three Major Fusion Transcripts
Authors:	Huang, YJ;Kuo, MC;Jaing, TH;Liu, HC;Yeh, TC;Chen, SH;Lin, TL;Yang, CP;Wang, PN;Sheen, JM;Chang, TK;Chang, CH;Hu, SF;Huang, TY;Wang, SC;Wu, KH;Chiou, SS;Hsiao, CC;Shih, LY
Date:	2021
Issue Date:	2022-08-05T09:41:44Z (UTC)
Publisher:	ELSEVIER SCIENCE INC
ISSN:	1525-1578
Abstract:	Two quantitative PCR (qPCR)ebased methods, for clonal immunoglobulin or T-cell receptor gene (Ig/ TCR) rearrangements and for fusion transcripts, are widely used for the measurement of minimal residual disease (MRD) in patients with B-precursor acute lymphoblastic leukemia (ALL). MRD of bone marrow samples from 165 patients carrying the three major fusion transcripts, including 74 BCR-ABL1, 54 ETV6RUNX1, and 37 TCF3-PBX1, was analyzed by using the two qPCR-based methods. The correlation coefficient of both methods was good for TCF3-PBX1 (R-2 Z 0.8088) and BCR-ABL1 (R-2 Z 0.8094) ALL and moderate for ETV6-RUNX1 (R-2 Z 0.5972). The concordance was perfect for TCF3-PBX1 ALL (97.2%), substantially concordant for ETV6-RUNX1 ALL (87.1%), and only moderate for BCR-ABL1 ALL (70.6%). The discordant MRD, positive for only one method with a difference greater than one log, was found in 4 of 93 samples (4.3%) with ETV6-RUNX1, 31 of 245 samples (12.7%) with BCR-ABL1, and none of TCF3PBX1 ALL. None of the eight non-transplanted patients with BCR-ABL1-MRD (+)/Ig/TCR-MRD (-) with a median follow-up time of 73.5 months had hematologic relapses. Our study showed an excellent MRD concordance between the two qPCR-based methods in TCF3-PBX1 ALL, whereas qPCR for Ig/TCR is more reliable in BCR-ABL1 ALL.
URI:	http://dx.doi.org/10.1016/j.jmoldx.2021.07.008 https://www.webofscience.com/wos/woscc/full-record/WOS:000704349600014 https://ir.csmu.edu.tw:8080/handle/310902500/23716
Relation:	JOURNAL OF MOLECULAR DIAGNOSTICS ,2021,v23,issue 10, P1373-1379
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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