Protective Effects of Ocimum gratissimumAqueous Extracts on HaCaT Cells Against UVC-Induced Inhibition of Cell Viability and Migration

doi:10.7150/ijms.54644

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/23667

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/23667

Title:	Protective Effects of Ocimum gratissimumAqueous Extracts on HaCaT Cells Against UVC-Induced Inhibition of Cell Viability and Migration
Authors:	Chang, SH;Liu, JY;Hsiao, MW;Yang, HL;Wang, GW;Ye, JC
Keywords:	Ultraviolet C;Skin cells;proliferation;migration;Ocimum gratissimum
Date:	2021
Issue Date:	2022-08-05T09:40:57Z (UTC)
Publisher:	IVYSPRING INT PUBL
ISSN:	1449-1907
Abstract:	Ultraviolet C (UVC) has been applied to treatment of infections in wounds for at least the last two decades, however, cells being treated can be damaged if exposure is prolonged, which calls for protective measures, such as drug or herbal pre-treatment, to minimize damage. Ocimum gratissimum contains plant polyphenols such as isoflavones and caffeic acid, which have antioxidant effects. We hypothesize that Ocimum gratissimum aqueous extracts (OGE) can inhibit UVC-induced oxidative damage on skin cells. In this study, HaCaT skin cells are used to test the protective effects of OGE on cell proliferation and migration after exposure to UVC radiation. Pretreatment with OGE (50 similar to 150 mu g/mL) before 40 J/m(2) UVC exposure was able to restore survival from 32.25% to between 46.77% and 68.00%, and 80 J/m(2) UVC exposure from 11.49% to between 19.07% and 43.04%. Morphological observation of primarily apoptotic cell death confirms the above findings. The flow cytometry analysis revealed that UVC increased the number of cells at the sub-G1 phase in a dose dependent manner, and when pre-treated with OGE the changes were partially reversed. Moreover, the wound healing test for observing migration showed that UVC 40-80 J/m(2) decreased cell migration to 47-28% activity and 100 mu g/mL OGE was able to restore cell activity to81-69% at day 3. Based on the above results, we suggest that OGE has a protective effect on UVC-induced inhibition of cell proliferation and migration of skin cells and thus has potential application in wound care.
URI:	http://dx.doi.org/10.7150/ijms.54644 https://www.webofscience.com/wos/woscc/full-record/WOS:000629084700020 https://ir.csmu.edu.tw:8080/handle/310902500/23667
Relation:	INTERNATIONAL JOURNAL OF MEDICAL SCIENCES ,2021,v18,issue 9, P2086-2092
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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