Differences in the Quantity and Composition of Extracellular Vesicles in the Aqueous Humor of Patients with Retinal Neovascular Diseases

doi:10.3390/diagnostics11071276

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/23615

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/23615

Title:	Differences in the Quantity and Composition of Extracellular Vesicles in the Aqueous Humor of Patients with Retinal Neovascular Diseases
Authors:	Hsiao, YP;Chen, C;Lee, CM;Chen, PY;Chung, WH;Wang, YP;Hung, YC;Cheng, CM;Chen, C;Ko, BH;Hsu, MY
Keywords:	extracellular vesicle;exosome;aqueous humor;nanoparticle tracking analysis;retinal neovascular disease;angiogenesis
Date:	2021
Issue Date:	2022-08-05T09:40:09Z (UTC)
Publisher:	MDPI
Abstract:	Extracellular vesicles (EVs) are secreted by various cells in the body fluid system and have been found to influence vessel formation and inflammatory responses in a variety of diseases. However, which EVs and their subtypes are involved in vascular retinal diseases is still unclear. Therefore, the aim of this study was to explore the particle distribution of EVs in retinal neovascular diseases, including age-related macular degeneration, polypoidal choroidal vasculopathy, and central retinal vein occlusion. The aqueous humor was harvested from 20 patients with different retinal neovascular diseases and six patients with cataracts as the control group. The particle distribution was analyzed using nanoparticle tracking analysis (NTA) and transmitting electron microscopy (TEM). The results revealed that the disease groups had large amounts of EVs and their subtypes compared to the control group. After isolating exosomes, a higher expression of CD81(+) exosomes was shown in the disease groups using flow cytometry. The exosomes were then further classified into three subtypes of exomeres, small exosomes, and large exosomes, and their amounts were shown to differ depending on the disease type. To the best of our knowledge, this is the first study to elucidate the dynamics of EVs in retinal neovascular diseases using clinical cases. Our findings demonstrated the possible functionality of microvesicles and exosomes, indicating the potential of exosomes in the diagnosis and therapy of retinal neovascular diseases.
URI:	http://dx.doi.org/10.3390/diagnostics11071276 https://www.webofscience.com/wos/woscc/full-record/WOS:000676917300001 https://ir.csmu.edu.tw:8080/handle/310902500/23615
Relation:	DIAGNOSTICS ,2021,v11,issue 7
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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