Magnolol ameliorates the accumulation of reactive oxidative stress and inflammation in diabetic periodontitis

doi:10.1016/j.jfma.2021.01.010

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/23605

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/23605

Title:	Magnolol ameliorates the accumulation of reactive oxidative stress and inflammation in diabetic periodontitis
Authors:	Liu, CM;Chen, SH;Liao, YW;Yu, CH;Yu, CC;Hsieh, PL
Keywords:	Periodontitis;Advanced glycation end products;Magnolol
Date:	2021
Issue Date:	2022-08-05T09:40:00Z (UTC)
Publisher:	ELSEVIER TAIWAN
ISSN:	0929-6646
Abstract:	Background/Purpose: Periodontal disease and diabetes mellitus (DM) are both chronic inflammatory and highly prevalent diseases. A large amount of evidence suggested that the accumulation of oxidative stress plays a significant role in the deterioration of both diseases. Magnolol has been known to possess anti-inflammatory and anti-oxidant activities in various tissues, but its effects on gingival cells under diabetic conditions have not been fully understood. Methods: We assessed the generation of reactive oxygen species (ROS), Transwell migration, and wound healing ability in response to the advanced glycation end products (AGEs) stimulation with or without Magnolol treatment. Subsequently, we examined the expression of Nrf2 and HO-1 to ascertain whether Magnolol was able to activate the anti-oxidant signaling. We also measured the secretion of IL-6 and IL-8, and conducted a knockdown experiment to elucidate the effect of Mrf2 on their secretion. Results: The AGEs-induced ROS was dose-dependently downregulated following the Magnolol treatment. Likewise, the reduced Transwell migration and wound healing ability were improved by various concentrations of Magnolol. Results from qRT-PCR indicated that the suppression of Nrf2 and HO-1 following AGEs stimulation was reversed by Magnolol. Also, the AGEselicited production of IL-6 and IL-8 was inhibited by Magnolol. Moreover, our results demonstrated that this anti-inflammatory effect was mediated by the upregulation of Nrf2. Conclusion: These findings showed that excessive AGEs in the gingiva may lead to the accumulation of ROS and pro-inflammatory cytokines. Supplement of Magnolol may be beneficial to improve the impaired wound healing and inflammation by upregulation of Nrf2 signaling for DM patients with periodontal disease. Copyright & ordf; 2021, Formosan Medical Association. Published by Elsevier Taiwan LLC. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
URI:	http://dx.doi.org/10.1016/j.jfma.2021.01.010 https://www.webofscience.com/wos/woscc/full-record/WOS:000669045200004 https://ir.csmu.edu.tw:8080/handle/310902500/23605
Relation:	JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION ,2021,v120,issue 7, P1452-1458
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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