Interleukin-1 beta-induced matrix metalloproteinase-3 via ERK1/2 pathway to promote mesenchymal stem cell migration

doi:10.1371/journal.pone.0252163

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/23533

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/23533

Title:	Interleukin-1 beta-induced matrix metalloproteinase-3 via ERK1/2 pathway to promote mesenchymal stem cell migration
Authors:	Chang, CH;Lin, YL;Tyan, YS;Chiu, YH;Liang, YH;Chen, CP;Wu, JC;Wang, HS
Date:	2021
Issue Date:	2022-08-05T09:38:49Z (UTC)
Publisher:	PUBLIC LIBRARY SCIENCE
ISSN:	1932-6203
Abstract:	Human umbilical cord Wharton's jelly derived mesenchymal stem cells (hUCMSCs), a source of cell therapy, have received a great deal of attention due to their homing or migrating ability in response to signals emanating from damaged sites. It has been found that IL-1 beta possesses the ability to induce the expression of matrix metalloproteinase-3 (MMP-3) in bone marrow MSCs. MMP-3 is involved in cell migration in various types of cells, including glioblastoma, vascular smooth muscle, and adult neural progenitor cells. In this study, we proposed that IL-1 beta influences hUCMSCs migration involving MMP-3. The expression level of MMP-3 in IL-1 beta -induced hUCMSCs was verified using cDNA microarray analysis, quantitative real-time PCR, ELISA and Western blot. Wound-healing and trans-well assay were used to investigate the cell migration and invasion ability of IL-1 beta -treated hUCMSCs. In addition, we pre-treated hUCMSCs with interleukin-1 receptor antagonist, MMP-3 inhibitors (ALX-260-165, UK 356618), or transfected with MMP-3 siRNA to confirm the role of MMP3 in IL-1 beta -induced cell migration. Our results showed that IL-1 beta induced MMP-3 expression is related to the migration of hUCMSCs. Moreover, extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) inhibitor U0126, p38 inhibitor SB205380, JNK inhibitor SP600125 and Akt inhibitor GSK 690693 decreased IL-1 beta -induced MMP-3 mRNA and protein expression. The migration and invasion ability analyses showed that these inhibitors attenuated the IL-1 beta -induced migration and invasion ability of hUCMSCs. In conclusion, we have found that IL-1 beta induces the expression of MMP-3 through ERK1/2, JNK, p38 MAPK and Akt signaling pathways to enhance the migration of hUCMSCs. These results provide further understanding of the mechanisms in IL-1 beta -induced hUCMSCs migration to injury sites.
URI:	http://dx.doi.org/10.1371/journal.pone.0252163 https://www.webofscience.com/wos/woscc/full-record/WOS:000664632300057 https://ir.csmu.edu.tw:8080/handle/310902500/23533
Relation:	PLOS ONE ,2021,v16,issue 5
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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