Arecoline enhances miR-21 to promote buccal mucosal fibroblasts activation

doi:10.1016/j.jfma.2020.10.019

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/23495

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题名:	Arecoline enhances miR-21 to promote buccal mucosal fibroblasts activation
作者:	Yang, HW;Yu, CC;Hsieh, PL;Liao, YW;Chu, PM;Yu, CH;Fang, CY
关键词:	Oral submucous fibrosis;MicroRNA-21;TGF-beta pathway;Myofibroblast
日期:	2021
上传时间:	2022-08-05T09:38:11Z (UTC)
出版者:	ELSEVIER TAIWAN
ISSN:	0929-6646
摘要:	Background/purpose: Oral submucous fibrosis (OSF) is an irreversible fibrosis disease and a potentially malignant disorder in the oral cavity. Various studies have shown that miR-21 was implicated in the fibrogenesis and carcinogenesis, but its functional role in the development of OSF has not been investigated. Methods: The expression levels of miR-21 in arecoline-stimulated normal buccal mucosal fibroblasts (BMFs) and OSF specimens were determined by qRT-PCR. Exogenous administration of TGF-beta and its inhibitor (SB431542) were utilized to examine the involvement of TGF-beta signaling in miR-21 alteration. Collagen gel contraction, transwell migration, and invasion assays were used to assess the myofibroblast activities. The relationship between alpha-SMA and miR-21 was calculated using the Pearson correlation coefficient. Results: MiR-21 expression was induced in BMFs by arecoline treatment in a dose-dependent manner. Our results showed that this upregulation was mediated by TGF-beta signaling. Subsequently, we demonstrated that the administration of the miR-21 inhibitor suppressed the arecoline-induced myofibroblast characteristics, including a higher collagen gel contractility and cell motility, in normal BMFs. Furthermore, inhibition of miR-21 was sufficient to attenuate the myofibroblast features in fibrotic BMFs. Besides, we showed that the expression of miR-21 was aberrantly upregulated in the OSF tissues and there was a positive correlation between miR-21 and myofibroblast marker, alpha-SMA. Conclusion: MiR-21 overexpression in OSF may be due to the stimulation of areca nut, which was mediated by the TGF-beta pathway. Our data suggested that the repression of miR-21 was a promising direction to palliate the development and progression of OSF. Copyright (C) 2020, Formosan Medical Association. Published by Elsevier Taiwan LLC.
URI:	http://dx.doi.org/10.1016/j.jfma.2020.10.019 https://www.webofscience.com/wos/woscc/full-record/WOS:000631881800008 https://ir.csmu.edu.tw:8080/handle/310902500/23495
關聯:	JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION ,2021,v120,issue 4, P1108-1113
显示于类别:	[中山醫學大學研究成果] 期刊論文

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