The Effects of Dimethylsulfoxide and Oxygen on DNA Damage Induction and Repair Outcomes for Cells Irradiated by 62 MeV Proton and 3.31 MeV Helium Ions

doi:10.3390/jpm11040286

CSMUIR > researcher portal > Artical > Item 310902500/23478

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/23478

Title:	The Effects of Dimethylsulfoxide and Oxygen on DNA Damage Induction and Repair Outcomes for Cells Irradiated by 62 MeV Proton and 3.31 MeV Helium Ions
Authors:	Chan, CC;Hsiao, YY
Keywords:	reactive oxygen species;dimethylsulfoxide;DNA damage induction;hypoxia
Date:	2021
Issue Date:	2022-08-05T09:37:57Z (UTC)
Publisher:	MDPI
Abstract:	Reactive oxygen species (ROS) play an essential role in radiation-induced indirect actions. In terms of DNA damage, double strand breaks (DSBs) have the greatest effects on the repair of DNA damage, cell survival and transformation. This study evaluated the biological effects of the presence of ROS and oxygen on DSB induction and mutation frequency. The relative biological effectiveness (RBE) and oxygen enhancement ratio (OER) of 62 MeV therapeutic proton beams and 3.31 MeV helium ions were calculated using Monte Carlo damage simulation (MCDS) software. Monte Carlo excision repair (MCER) simulations were used to calculate the repair outcomes (mutation frequency). The RBE values of proton beams decreased to 0.75 in the presence of 0.4 M dimethylsulfoxide (DMSO) and then increases to 0.9 in the presence of 2 M DMSO while the RBE values of 3.31 MeV helium ions increased from 2.9 to 5.7 (0-2 M). The mutation frequency of proton beams also decreased from 0.008-0.065 to 0.004-0.034 per cell per Gy by the addition of 2 M DMSO, indicating that ROS affects both DSB induction and repair outcomes. These results show that the combined use of DMSO in normal tissues and an increased dose in tumor regions increases treatment efficiency.
URI:	http://dx.doi.org/10.3390/jpm11040286 https://www.webofscience.com/wos/woscc/full-record/WOS:000643106800001 https://ir.csmu.edu.tw:8080/handle/310902500/23478
Relation:	JOURNAL OF PERSONALIZED MEDICINE ,2021,v11,issue 4
Appears in Collections:	[researcher portal] Artical

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	168	View/Open

Loading...