The mechanism of anticancer activity of the new synthesized compound-6,7-Methylenedioxy-4-(2,4-dimethoxyphenyl) quinolin-2(1H)-one(12e) in human ovarian cancer cell lines

doi:10.1016/j.tjog.2021.01.002

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/23391

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/23391

Title:	The mechanism of anticancer activity of the new synthesized compound-6,7-Methylenedioxy-4-(2,4-dimethoxyphenyl) quinolin-2(1H)-one(12e) in human ovarian cancer cell lines
Authors:	Chan, HC;Liao, YH;Chang, HW;Liu, CH
Keywords:	4-Phenylquinolin-2(1H)-one;Anticancer agent;Ovarian cancer;Apoptosis
Date:	2021
Issue Date:	2022-08-05T09:36:31Z (UTC)
Publisher:	ELSEVIER TAIWAN
ISSN:	1028-4559
Abstract:	Objective: Ovarian cancer is the most lethal of the gynecologic malignancies. Most women have advanced disease at diagnosis and require extensive debulking surgery and aggressive chemotherapy. Induction of apoptosis in cancer cells has been used as an important approach for cancer therapy. We examined the anticancer effect of 6,7-methylenedioxy-4-(2,4-dimethoxyphenyl)quinolin-2(1H)-one (12e) in human ovarian cancer cell line. Materials and methods: The 6,7-methylenedioxy-4-(2,4-dimethoxyphenyl) quinolin-2 (1H)-one (12e) was synthesized and provided by Dr. Li-Jiau Huang of China Medical University. Cell viability analysis showed that 12e inhibited cell growth and induced cell death in time-and dose-dependent manners. In order to study the underlying cell death mechanism, 2774 and SKOV3 cells treated with 12e were studied by morphology, DAPI/TUNEL double staining, DNA gel electrophoresis. To search the mechanisms of anti proliferative effect of 12e, cell cycle analysis was performed. Changes in proteins related to cell death were analyzed by Western blot. Results: 12e significantly induced apoptosis evidenced by morphological changes, TUNEL-DAPI double staining and DNA fragmentation. Western blot analysis demonstrated that the protein level of Bcl-2 was decreased after treatment with 12e, while the level of p53 and Bax was increased. 12e treatment resulted in G2/M arrest through down modulation of cyclin B1 and cdk1. Conclusion: These results suggested that 12e-induced growth inhibition was associated with cell cycle arrest and apoptosis. (c) 2021 Taiwan Association of Obstetrics & Gynecology. Publishing services by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
URI:	http://dx.doi.org/10.1016/j.tjog.2021.01.002 https://www.webofscience.com/wos/woscc/full-record/WOS:000631779400011 https://ir.csmu.edu.tw:8080/handle/310902500/23391
Relation:	TAIWANESE JOURNAL OF OBSTETRICS & GYNECOLOGY ,2021,v60,issue 2, P266-272
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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