血清中骨橋蛋白濃度應用於骨質疏鬆症臨床診斷之可能性評估

CSMUIR > Medical College > Institute of Medicine > Electronic Theses of Dissertations > Item 310902500/230

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/230

Title:	血清中骨橋蛋白濃度應用於骨質疏鬆症臨床診斷之可能性評估 Application of plasma osteopontin concentrations in clinical diagnosis of osteoporosis
Authors:	蔡文龍 Wen-Lung Tsai
Contributors:	中山醫學大學：醫學研究所周明智鄭雅文
Keywords:	骨橋蛋白骨質疏鬆症 Osteopontin Osteoporosis
Date:	2006/06/22
Issue Date:	2009-11-26T01:55:54Z (UTC)
Abstract:	骨質疏鬆症已是世界性的重要流行病。易導致各部位發生骨折，而其中尤以脊椎體及髖部骨折最為嚴重。台灣女性老年人骨質疏鬆症的流行率為18%，而男性為12%。若依目前台灣婦女平均壽命為79.3歲而言，大約三分之一的台灣婦女在一生中會發生一次脊椎體、髖部或腕部之骨折;男性也約有五分之一的風險。骨橋蛋白（osteopontin，OPN）是一種酸性，合鈣離子的醣磷酸蛋白質，存在於所有體液及細胞外間質，是由骨細胞所產生的一種蛋白，並且認為與骨的代謝調節有機能上的重要性。OPN並非正常骨發展所需要，但是卻與骨骼再塑 (bone remodeling) 及骨的強度(strength) 有關。過去有動物實驗的研究指出OPN與骨質疏鬆症的骨骼溶蝕吸收 (resorption) 的發作 (onset) 效應有關，但在人類OPN濃度與骨質疏鬆症的相關性並不清楚。本研究收集具骨質疏鬆症家族史的實驗組血清檢體共45人，不具骨質疏鬆症家族史的對照組5人，利用酵素免疫結合吸附法 (ELISA; enzyme-link immunosorbent assay) 分析其血清中OPN濃度。結果發現年齡越高OPN值越高，兩者具統計上的相關性 (P=0.026)，骨密度T-score小於-2.5時血清中的OPN濃度高於T-score大於-1時，(T-score<-2.5, OPN=11.76±5.44 ng/ml; T-score>-1, OPN=77±5.32ng/ml)，其餘臨床因子則無統計上的意義。這結果顯示OPN值越高可能有較高罹患骨質疏鬆症機率的危險性。因此本研究在未來擬增加無骨質疏鬆症家族史的對照組，以釐清血清中OPN濃度應用於骨質疏鬆症早期診斷的可行性。 Osteoporosis is one of the most common world diseases, the results of osteoporosis are multiple bony fractures. The most severe fractures are spinal vertebral fracture and hip fracture. The osteoporosis incidence rate of Taiwan female is 18%, Taiwan male is 12%. According to the life span of Taiwan female is 78.3 years old, about one third of female ,will occur spinal, hip or radial fracture in the life, and one fifth of male have the same risk rate. OPN is a calcium-binding phosphoprotein which exists in the body fluid and extracellular interstitial fluid. The role of osteopontin (OPN), a protein produced by bone cells and regarded as functionally important in the regulation of bone metabolism. OPN is not required for normal bone development, but is required for certain types of bone remodeling and it strengthens. In the past, some animal study had evidence that the functions of OPN have its effects on the onset of the bone resorption associated with osteoporosis. While no information exists regarding OPN deficiency in humans, This study correct experimental serums of total 45 persons with osteoporotic family history and another 5 persons without osteoportic family history ,using ELISA( enzyme-link immunosorbent assay)method to check up the OPN concentration level. The result finds the age has positive correlation with OPN concentration level, it means with the older age, with the higher OPN level (P value =0.026). The plasma OPN level in the group of T-score <-2.5 (11.76±5.44 ng/ml) was significant higher than the level of T-score >-1 (9.77±5.32ng/ml). Other clinic factors had no statically significant. The plasma OPN level in the contrast group without osteoporosis family history was 5.37±1.81ng/ml . With the higher level of OPN, there was higher osteoporosis risk incidence. Therefore this study in the future will increase contrast sample size without ostoporotic family history to evidence the possibility in application of plasma osteopontin concertrations in clinical diagnosis of osteoporosis. with the older age, with the higher OPN level (P value =0.026). The plasma OPN level in the group of T-score <-2.5 (11.76±5.44 ng/ml) was significant higher than the level of T-score >-1 (9.77±5.32ng/ml). Other clinic factors had no statically significant. The plasma OPN level in the contrast group without osteoporosis family history was 5.37±1.81ng/ml . With the higher level of OPN, there was higher osteoporosis risk incidence. .Therefore this study in the future will increase contrast sample size without ostoporotic family history to evidence the possibility in application of plasma osteopontin concertrations in clinical diagnosis of osteoporosis.
URI:	http://140.128.138.153:8080/handle/310902500/230
Appears in Collections:	[Institute of Medicine] Electronic Theses of Dissertations

Files in This Item:

File	Description	Size	Format
醫研所177.pdf	碩士論文	420Kb	Adobe PDF	536	View/Open

Loading...