Umbilical Cord Mesenchymal Stromal Cell-Derived Exosomes Rescue the Loss of Outer Hair Cells and Repair Cochlear Damage in Cisplatin-Injected Mice

doi:doi.org/10.3390/ijms22136664

中山醫學大學機構典藏 CSMUIR > 醫學院 > 醫學系 > 期刊論文 > Item 310902500/22397

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题名:	Umbilical Cord Mesenchymal Stromal Cell-Derived Exosomes Rescue the Loss of Outer Hair Cells and Repair Cochlear Damage in Cisplatin-Injected Mice
作者:	Tsai, Stella Chin-Shaw;Yang, Kuender D.;Chang, Kuang-Hsi;Lin, Frank Cheau-Feng;Chou, Ruey-Hwang;Li, Min-Chih;Cheng, Ching-Chang;Kao, Chien-Yu;Chen, Chie-Pein;Lin, Hung-Ching;Hsu, Yi-Chao
关键词:	mesenchymal stromal cells;cisplatin;hair cells;inner ear;ototoxicity;extracellular vesicles;exosomes
日期:	2021-07
上传时间:	2022-06-07T03:20:00Z (UTC)
出版者:	MDPI
摘要:	Umbilical cord-derived mesenchymal stromal cells (UCMSCs) have potential applications in regenerative medicine. UCMSCs have been demonstrated to repair tissue damage in many inflammatory and degenerative diseases. We have previously shown that UCMSC exosomes reduce nerve injury-induced pain in rats. In this study, we characterized UCMSC exosomes using RNA sequencing and proteomic analyses and investigated their protective effects on cisplatin-induced hearing loss in mice. Two independent experiments were designed to investigate the protective effects on cisplatin-induced hearing loss in mice: (i) chronic intraperitoneal cisplatin administration (4 mg/kg) once per day for 5 consecutive days and intraperitoneal UCMSC exosome (1.2 μg/μL) injection at the same time point; and (ii) UCMSC exosome (1.2 μg/μL) injection through a round window niche 3 days after chronic cisplatin administration. Our data suggest that UCMSC exosomes exert protective effects in vivo. The post-traumatic administration of UCMSC exosomes significantly improved hearing loss and rescued the loss of cochlear hair cells in mice receiving chronic cisplatin injection. Neuropathological gene panel analyses further revealed the UCMSC exosomes treatment led to beneficial changes in the expression levels of many genes in the cochlear tissues of cisplatin-injected mice. In conclusion, UCMSC exosomes exerted protective effects in treating ototoxicity-induced hearing loss by promoting tissue remodeling and repair.
URI:	https://ir.csmu.edu.tw:8080/handle/310902500/22397
關聯:	Int J Mol Sci, 22(13), 6664.
显示于类别:	[醫學系] 期刊論文

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