Er:YAG laser promotes proliferation and wound healing capacity of human periodontal ligament fibroblasts through Galectin-7 induction

doi:doi.org/10.1016/j.jfma.2020.06.005

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 口腔醫學研究所 > 期刊論文 > Item 310902500/22357

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/22357

Title:	Er:YAG laser promotes proliferation and wound healing capacity of human periodontal ligament fibroblasts through Galectin-7 induction
Authors:	Lin, Taichen;Yu, Cheng-Chia;Liu, Chia-Ming;Hsieh, Pei-Ling;Liao, Yi-Wen;Yu, Chuan-Hang;Chen, Chun-Jung
Keywords:	Er:YAG laser;LGALS7;Periodontal ligament fibroblasts.
Date:	2021-01
Issue Date:	2022-05-30T03:22:42Z (UTC)
Publisher:	Elsevier Inc.
Abstract:	Background/purpose Among various dental lasers, the erbium-doped yttrium-aluminum-garnet (Er:YAG) laser has great potential for periodontal treatment including soft and hard tissue ablation with minimal thermal side effects under suitable energy densities and it has multiple effects on tissues for wound-healing benefits. In the present study, we sought to reveal the molecular mechanism underlying the impact of Er:YAG laser on PDL fibroblasts. Methods Cells were irradiated by a Er:YAG laser with various energy densities (3.6–6.3 J/cm 2 ). MTT assay was used for cell proliferation, and the transwell system was employed for migration and invasion abilities. The wound healing capacity was evaluated by a scratch assay. After confirming these effects, qRT-PCR and western blotting analysis was applied to identify the differentially galectin-7 expression in the irradiated cells. Knockdown experiments were conducted to reveal the functional role of galectin-7 in the modulation of Er:YAG laser-mediated effects. Results 4.2 J/cm 2 was the lowest energy density to induce the optimal cell proliferation, migration and invasion abilities. In the group of upregulated genes, galectin-7 was selected for further examination and its elevation after Er:YAG laser treatment was validated by RT-PCR and Western blot. We demonstrated that silence of galectin-7 abrogated the effects of Er:YAG laser on cell proliferation, migration ad invasion, suggesting the Er:YAG laser promoted these effects through induction of galectin-7. Conclusion These findings indicated that Er:YAG laser may accelerate the regeneration process in periodontal tissues through enhancement of their proliferative and mobile activities. Additionally, the significance of galectin-7 in the Er:YAG laser-elicited benefits was demonstrated.
URI:	https://ir.csmu.edu.tw:8080/handle/310902500/22357
Relation:	J Formos Med Assoc,120(1),388-394
Appears in Collections:	[口腔醫學研究所] 期刊論文

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