miR-1246 as a therapeutic target in oral submucosa fibrosis pathogenesis

doi:doi.org/10.1016/j.jfma.2019.02.014

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 口腔醫學研究所 > 期刊論文 > Item 310902500/22351

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题名:	miR-1246 as a therapeutic target in oral submucosa fibrosis pathogenesis
作者:	Chia-Ming Liu;Yi-Wen Liao;Pei-Ling Hsieh;Chuan-Hang Yu;Pin Ju Chueh;Taichen Lin;Po-Yu Yang;Cheng-Chia Yu;Ming-Yung Chou
关键词:	Buccal mucosal fibroblasts;MicroRNA-1246;Oral submucous fibrosis;Type I collagen
日期:	2019-07
上传时间:	2022-05-30T02:58:34Z (UTC)
出版者:	Elsevier Inc.
摘要:	Background/purpose: Oral submucous fibrosis (OSF) is a precancerous condition of oral cancer with a complex etiology. Our previous work has demonstrated that non-coding RNA miR-1246 contributes to the cancer stemness of oral cancer. In the current study, we sought to investigate the effect of the inhibition of miR-1246 on the oral fibrogenesis. Methods: The expression levels of miR-1246 in OSF tissues and fibrotic buccal mucosal fibroblasts (fBMFs) were examined by qRT-PCR. Collagen gel contraction and migration assays were conducted to evaluate the myofibroblast activities. The relationship between miR-1246 and type I collagen was assessed and the protein expression of type I collagen was determined by Western blot. Results: MiR-1246 expression was upregulated in both OSF specimen and fBMFs compared to the normal counterparts. Inhibition of miR-1246 successfully suppressed the myofibroblast activities, including collagen gel contractility and migration capacity. Moreover, the expression of miR-1246 was positively correlated with type I collagen and the expression of type I collagen was abrogated by repression of miR-1246. Conclusion: MiR-1246 is not only critical to the maintenance of oral stemness but also important to the activation of myofibroblasts. Our results showed that miR-1246 is positively associated with the type I collagen, which may be a downstream effector of miR-1246 and responsible for the fibrosis effect on fBMFs.
URI:	https://ir.csmu.edu.tw:8080/handle/310902500/22351
關聯:	J Formos Med Assoc,118(7),1093-1098
显示于类别:	[口腔醫學研究所] 期刊論文

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