Docosahexaenoic acid increases the expression of oxidative stress-induced growth inhibitor 1 through the PI3K/Akt/Nrf2 signaling pathway in breast cancer cells

doi:doi.org/10.1016/j.fct.2017.08.010

中山醫學大學機構典藏 CSMUIR > 健康管理學院 > 營養學系暨碩士班 > 期刊論文 > Item 310902500/22347

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题名:	Docosahexaenoic acid increases the expression of oxidative stress-induced growth inhibitor 1 through the PI3K/Akt/Nrf2 signaling pathway in breast cancer cells
作者:	Tsai, Chia-Han;Shen, You-Cheng;Chen, Haw-Wen;Liu, Kai-Li;Chang, Jer-Wei;Chen, Pei-Yin;Lin, Chen-Yu;Yao, Hsien-Tsung;Li, Chien-Chun
贡献者:	中山醫學大學;營養系
关键词:	Apoptosis;Breast cancer;Docosahexaenoic acid;Nrf2;OSGIN1
日期:	2017-10
上传时间:	2022-05-17T03:52:53Z (UTC)
出版者:	Elsevier Inc.
摘要:	Oxidative stress-induced growth inhibitor 1 (OSGIN1), a tumor suppressor, inhibits cell proliferation and induces cell death. N-6 and n-3 PUFAs protect against breast cancer, but the molecular mechanisms of this effect are not clear. We investigated the effect of n-6 and n-3 PUFAs on OSGIN1 expression and whether OSGIN1 is involved in PUFA-induced apoptosis in breast cancer cells. We used 100 μM of n-6 PUFAs including arachidonic acid, linoleic acid, and gamma-linolenic acid and n-3 PUFAs including alpha-linolenic acid, eicosapentaenoic acid, and docosahexaenoic acid (DHA). Only DHA significantly induced OSGIN1 protein and mRNA expression. DHA triggered reactive oxygen species (ROS) generation and nuclear translocation of Nrf2. LY294002, a PI3K inhibitor, suppressed DHA-induced OSGIN1 protein expression and nuclear accumulation of Nrf2. Nrf2 knockdown attenuated DHA-induced OSGIN1 expression. N-Acetyl-l-cysteine, a ROS scavenger, abrogated the DHA-induced increases in Akt phosphorylation, Nrf2 nuclear accumulation, and OSGIN1 expression. DHA induced the Bax/Bcl-2 ratio, mitochondrial accumulation of OSGIN1 and p53, and cytochrome c release; knockdown of OSGIN1 diminished these effects. In conclusion, induction of OSGIN1 by DHA is at least partially associated with increased ROS production, which activates PI3K/Akt/Nrf2 signaling. Induction of OSGIN1 may be involved in DHA-induced apoptosis in breast cancer cells.
URI:	https://ir.csmu.edu.tw:8080/handle/310902500/22347
關聯:	Food Chem Toxicol,108(Pt A),276-288
显示于类别:	[營養學系暨碩士班] 期刊論文

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