Naringenin inhibited migration and invasion of glioblastoma cells through multiple mechanisms

doi:doi.org/10.1002/tox.22677

中山醫學大學機構典藏 CSMUIR > 醫學院 > 醫學系 > 期刊論文 > Item 310902500/21624

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/21624

Title:	Naringenin inhibited migration and invasion of glioblastoma cells through multiple mechanisms
Authors:	Yen-Yu Chen;Yuh-Ming Chang;Kuan-Yi Wang;Pei-Ni Chen;You-Cheng Hseu;Ke-Min Chen;Kun-Tu Yeh;Chih-Jung Chen;Li-Sung Hsu
Keywords:	glioblastoma;invasion;migration;naringenin.
Date:	2018-11-15
Issue Date:	2021-08-17T01:58:24Z (UTC)
Publisher:	John Wiley & Sons, Inc.
Abstract:	Glioblastoma (GBM) is the most mortality brain cancer in the world. Due to high invasion and drug resistance cause the poor prognosis of GBM. Naringenin, an ingredient of citrus, exhibits many cellular functions such as antioxidant, anti-inflammation, and anticancer. Naringenin inhibits the migration of bladder and lung cancer via modulation of MMP-2 and/or MMP-9 activities, Naringenin inhibits migration and trigger apoptosis in gastric cancer cells through downregulation of AKT pathway. However, the effects of naringenin in GBM still remain to be elucidated. In this study, we reveal the molecular mechanisms of naringenin in the inhibition of migration and invasion in GBM. No overt alternation of cell proliferation was found in of GBM 8901 cells treated with different concentration of naringenin. Slight decreased cell viability was found in GBM 8401 cell treated with 200 and 300 μM naringenin. Significant reduction of migration and invasion as assayed by Boyden chamber analysis was found in of GBM cells treated with 100, 200, and 300 μM naringenin. Zymography analysis also revealed that the activities of MMP-2 and MMP-9 of GBM cells were significantly inhibited in response to 100, 200, or 300 μM naringenin treatment. Proteins of MMP-2 and MMP-9 were downregulated in naringenin treated GBM cells. In addition, naringenin also attenuated the activities of ERK and p38. Naringenin decreased mesenchymal markers (snail and slug) expression as revealed by Western blot analysis. Taken together, our findings indicated that naringenin eliminated the migration and invasion of GBM cells through multiple mechanisms including inhibition of MMPs, ERK, and p38 activities and modulation of EMT markers. Our results also suggested that naringenin may be a potential agent to prevent metastasis of GBM.
URI:	https://ir.csmu.edu.tw:8080/handle/310902500/21624
Relation:	Environmental toxicology, Volume34, Issue3,Pages 233-239
Appears in Collections:	[醫學系] 期刊論文

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