近年來組織工程的興起，促使生物陶瓷相關研究快速發展。以矽酸鈣（Calcium silicate：CS）為基底的陶瓷材料因具備良好的骨誘導能力，因而受到關注。過去實驗室自行開發出含鎂矽酸鈣（MgCS）及含鍶矽酸鈣（SrCS）等生物陶瓷。經實驗證實MgCS具有良好的生物降解能力，而SrCS具較高的機械性質，且兩者都具有刺激幹細胞的增殖及分化的能力。本研究將透過三維列印技術製備陶瓷支架，透過支架結構設計製作出同時具有含鎂及含鍶矽酸鈣的複合支架（MSM），並對MgCS、SrCS及MSM支架進行機械性質、降解性能及生物活性等相關測試，希望鎂離子跟鍶離子同時釋放來刺激骨組織再生，並透過支架的材料設計改善MgCS植入體內後機械性質不足的問題。實驗結果顯示MSM支架的機械性質介於MgCS跟SrCS之間，而在生物活性的部分，雖然MSM支架對於細胞生長並沒有顯著的差異，但在骨分化實驗中，能夠發現有較好的鹼性磷酸酶活性及更多的鈣沉積於支架表面。在動物實驗的部分使用紐西蘭大白兔的脛骨作為實驗模型，從組織切片上能明顯的觀察到MSM支架相較於MgCS及SrCS支架皆呈現了較好的骨生成效果，並且能觀察到較多的血管新生組織。因此本研究證實由三維列印製含MgCS跟SrCS多孔支架，除了能夠改善機械性質不足的缺點，更是能夠藉由不同材料所釋放的離子來促進不同的組織再生，必能提升未來於臨床上的使用可行性。
In recent years, due to the rise of tissue engineering, the rapid development of bioceramics has been promoted. calcium silicate (CS) based ceramic materials have received attention due to their good osteoinductive ability. in the past, the laboratory developed magnesium-containing calcium silicate (MgCS) and strontium-containing calcium silicate (SrCS). it has been confirmed by experiments that MgCS has good biodegradability, while SrCS has high mechanical properties, both of which have the effect of stimulating osteoblast proliferation and differentiation. the composite scaffold (MSM) with magnesium and strontium containing calcium silicate is designed through the structure of the scaffold. the mechanical properties, degradation properties and biological activities of the MgCS, SrCS and MSM scaffolds were tested. it is desirable that the release of magnesium ions and strontium ions simultaneously stimulate bone tissue regeneration, and the structural design of the scaffold improves the strength of the MgCS scaffold. the experimental results confirmed that the three-dimensionally printed MSM scaffold has better mechanical properties than the MgCS scaffold. although MSM scaffolds did not significantly promote cell growth. however, alkaline phosphatase activity and more calcium deposition were found on the surface of the scaffold.
