Deep sea minerals ameliorate diabetic-induced inflammation via inhibition of TNFα signaling pathways

doi:10.1002/tox.22882

中山醫學大學機構典藏 CSMUIR > 醫學科技學院 > 醫學檢驗暨生物技術學系暨碩士班 > 期刊論文 > Item 310902500/20655

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/20655

Title:	Deep sea minerals ameliorate diabetic-induced inflammation via inhibition of TNFα signaling pathways
Authors:	Lu, C.-H. Ou, H.-C. Day, C.-H. Chen, H.-I. Pai, P.-Y. Lee, C.-Y. Chen, R.-J. Chang, R.-L. Padma Viswanadha, V. Hsieh, D.J.-Y. Huang, C.-Y.
Date:	2020-04
Issue Date:	2020-03-25T04:34:20Z (UTC)
Publisher:	Environmental Toxicology
ISSN:	1520-4081
Abstract:	It has been well-documented that the consumption of deep sea water (DSW) has beneficial effects on myocardial hypertrophy and cardiac apoptosis induced by hypercholesterolemia. However, the molecular mechanisms for the anti-inflammatory effects of DSW on diabetic cardiomyopathy are still largely unclear. The main purpose of this present study was to test the hypothesis that DSW exerts anti-inflammatory effects through the suppression of the TNF-α-mediated signaling pathways. IP injection of streptozotocin (STZ) at the dose of 65 mg/kg was used to establish a diabetes rat model. DSW mineral extracts that diluted in desalinated water were prepared in three different dosages and administered to the rats through gavages for 4 weeks. These dosages are DSW-1X (equivalent to 37 mg Mg2+/kg/day), 2X (equivalent to 74 mg Mg2+/kg/day) and 3X (equivalent to 111 mg Mg2+ mg/kg/day). Immunofluorescence staining and Western blot showed that the protein expression level of TNF-α was markedly higher in the STZ-induced diabetic rat hearts than in the control group. Consequently, the phosphorylation levels of the TNF-α-modulated downstream signaling molecules and P38 mitogen-activated protein kinases (MAPKs) were notably elevated in heart tissues of STZ-induced diabetes. These higher phosphorylation levels subsequently upregulated NF-κB-modulated inflammatory mediators, such as cyclooxygenase (COX)-II and inducible nitric oxide synthase (iNOS). However, treatment with DSW as well as MgSO4, the main mineral in DSW, significantly reversed all the alterations. These findings suggest that DSW has potential as a therapeutic agent for preventing diabetes-related cardiovascular diseases. © 2019 Wiley Periodicals, Inc.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/20655
Relation:	Environmental Toxicology, Volume 35, Issue 4, 468-477
Appears in Collections:	[醫學檢驗暨生物技術學系暨碩士班] 期刊論文

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