Rutin Inhibits Oleic Acid Induced Lipid Accumulation via Reducing Lipogenesis and Oxidative Stress in Hepatocarcinoma Cells

doi:10.1111/j.1750-3841.2010.02033.x

中山醫學大學機構典藏 CSMUIR > 醫學院 > 醫學系 > 期刊論文 > Item 310902500/20475

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/20475

Title:	Rutin Inhibits Oleic Acid Induced Lipid Accumulation via Reducing Lipogenesis and Oxidative Stress in Hepatocarcinoma Cells
Authors:	Wu, Cheng‐Hsun Lin, Ming‐Cheng Wang, Hsueh‐Chun Yang, Mon‐Yuan Jou, Ming‐Jia Wang, Chau‐Jong
Contributors:	中山醫學大學醫學系
Keywords:	antioxidant enzymes;hepatic lipogenesis;lipid accumulation;rutin
Date:	2011-03
Issue Date:	2019-11-21T04:19:38Z (UTC)
Publisher:	Journal of Food Science
ISSN:	0022-1147
Abstract:	Excessive lipid accumulation within liver has been proposed to cause obesity, hyperlipidemia, diabetes, and fatty liver disease. Rutin, a common dietary flavonoid that is consumed in fruits, vegetables, and plant‐derived beverages, has various biological functions, including antioxidant, anti‐inflammatory, and anticancer effects. However, a hypolipidemic effect of rutin on fatty liver disease has not been reported. In this study, we examined the effect of rutin on reducing lipid accumulation in hepatic cells. Hepatocytes were treated with oleic acid (OA) containing with or without rutin to observe the lipid accumulation by Nile red stain. The result showed rutin suppressed OA‐induced lipid accumulation and increased adenosine 5′‐monophosphate (AMP)‐activated protein kinase (AMPK) activity in hepatocytes. The expression of critical molecule involved in lipid synthesis, sterol regulatory element binding proteins‐1 (SREBP‐1), was attenuated in rutin‐treated cells. Moreover, long‐term incubation of rutin inhibited the transcriptions of 3‐hydroxy‐3‐methylglutaryl‐coenzyme A (HMG‐CoA) reductase (HMGCR), glycerol‐3‐phosphate acyltransferase (GPAT), fatty acid synthase (FAS), and acetyl‐coenzyme carboxylase (ACC). Besides, we also found out the antioxidative effect of rutin by increasing the expression of peroxisome proliferator‐activated receptor (PPAR)‐α and antioxidative enzymes. Taken together, our findings suggest rutin could attenuate lipid accumulation by decreasing lipogenesis and oxidative stress in hepatocyte.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/20475
Relation:	Journal of Food Science, Volume 76, Issue 2,Pages T65-T72
Appears in Collections:	[醫學系] 期刊論文

Files in This Item:

File	Description	Size	Format
j.1750-3841.2010.02033.x.pdf		515Kb	Adobe PDF	154	View/Open

View Licence

Loading...