Dioscorea nipponica Attenuates Migration and Invasion by Inhibition of Urokinase-Type Plasminogen Activator through Involving PI3K/Akt and Transcriptional Inhibition of NF-κ B and SP-1 in Hepatocellular Carcinoma

doi:10.1142/S0192415X16500129

中山醫學大學機構典藏 CSMUIR > 醫學科技學院 > 醫學檢驗暨生物技術學系暨碩士班 > 期刊論文 > Item 310902500/20262

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题名:	Dioscorea nipponica Attenuates Migration and Invasion by Inhibition of Urokinase-Type Plasminogen Activator through Involving PI3K/Akt and Transcriptional Inhibition of NF-κ B and SP-1 in Hepatocellular Carcinoma
作者:	Hsieh, M.-J. Yeh, C.-B. Chiou, H.-L. Hsieh, M.-C. Yang, S.-F.
关键词:	Dioscorea nipponica Makino;Hepatoma;Invasion;Migration;MMP-9;u-PA
日期:	2016-02
上传时间:	2019-07-16T07:56:34Z (UTC)
出版者:	American Journal of Chinese Medicine
ISSN:	0192-415X
摘要:	High mortality and morbidity rates for hepatocellular carcinoma (HCC) in Taiwan primarily result from uncontrolled tumor metastasis. In our previous studies, we have reported that Dioscorea nipponica Makino extract (DNE) has anti-metastasis effects on human oral cancer cells. However, the effect of DNE on hepatoma metastasis have not been thoroughly investigated and remains poorly understood. To determine the effects of DNE on the migration and invasion in HCC cells we used a wound healing model, Boyden chamber assays, gelatin/casein zymography and Western blotting. Transcriptional levels of matrix metalloproteinase-9 (MMP-9) and urokinase-type plasminogen activator (u-PA) were detected by real-time PCR and promoter assays. In this study, DNE treatment significantly inhibited the migration/invasion capacities of Huh7 cell lines. The results of gelatin/casein zymography and Western blotting revealed that the activities and protein levels of the MMP-9 and u-PA were inhibited by DNE. Tests of the mRNA levels, real-time PCR, and promoter assays evaluated the inhibitory effects of DNE on u-PA expression in human hepatoma cells. A chromatin immunoprecipitation (ChIP) assay showed not only that DNE inhibits u-PA expression, but also the inhibitory effects were associated with the down-regulation of the transcription factors of NF-κB and SP-1 signaling pathways. Western blot analysis also showed that DNE inhibits PI3K and phosphorylation of Akt. In conclusion, these results show that u-PA expression may be a potent therapeutic target in the DNE-mediated suppression of HCC invasion/migration. DNE may have potential use as a chemo-preventive agent against liver cancer metastasis. © 2016 World Scientific Publishing Company.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/20262
關聯:	American Journal of Chinese Medicine, Vol.44, Issue 1, 177-195
显示于类别:	[醫學檢驗暨生物技術學系暨碩士班] 期刊論文

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