Elevation of Twist expression by arecoline contributes to the pathogenesis of oral submucous fibrosis

doi:10.1016/j.jfma.2015.05.009

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 口腔醫學研究所 > 期刊論文 > Item 310902500/20222

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题名:	Elevation of Twist expression by arecoline contributes to the pathogenesis of oral submucous fibrosis
作者:	Lee, Yu-Hsien Yang, Li-Chiu Hu, Fang-Wei Peng, Chih-Yu Yu, Chuan-Hang Yu, Cheng-Chia
贡献者:	中山醫學大學口腔醫學研究所
关键词:	arecoline;myofibroblasts;oral submucous fibrosis;Twist
日期:	2016-05
上传时间:	2019-06-11T03:37:31Z (UTC)
出版者:	Journal of the Formosan Medical Association
ISSN:	0929-6646
摘要:	Background/purpose Oral submucous fibrosis (OSF), a chronic progressive scarring disease, has been considered as a precancerous condition of oral mucosa. In this study, we investigated the functional role of Twist, an epithelial-mesenchymal transition (EMT) transcriptional factor, in myofibroblastic differentiation activity of OSF. Methods Arecoline, a major areca nut alkaloid, was used to explore whether expression of Twist could be changed dose-dependently in human primary buccal mucosal fibroblasts (BMFs). Collagen gel contraction and migration capability in arecoline-stimulated BMFs and primary oral submucous fibrosis-derived fibroblasts (OSFs) with Twist knockdown was presented. Results We observed that the treatment of arecoline dose-dependently increased Twist expression transcript and protein levels in BMFs. The myofibroblast activity including collagen gel contraction and migration capability also induced by arecoline, while knockdown of Twist reversed these phenomena. Importantly, inhibition of Twist led to the suppression collagen contraction and wound healing capability of primary cultivated OSFs. Clinically, Twist transcript and protein expression was higher in areca quid chewing-associated OSF tissues than in normal oral mucosa tissues. Conclusion This evidence suggests that upregulation of Twist might be involved in the pathogenesis of areca quid-associated OSF through dysregulation of myofibroblast activity.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/20222
關聯:	Journal of the Formosan Medical Association Vol.115, Issue 5, Pages 311-317
显示于类别:	[口腔醫學研究所] 期刊論文

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