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Please use this identifier to cite or link to this item:
https://ir.csmu.edu.tw:8080/ir/handle/310902500/19280
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Title: | The attenuation of renal fibrosis by histone deacetylase inhibitors is associated with the plasticity of FOXP3+IL-17+ T cells |
Authors: | WP, Wu YG, Tsai TY, Lin MJ, Wu CY, Lin |
Contributors: | 中山醫學大學 |
Keywords: | FOXP3+ IL-17+ T cells;Renal fibrosis;Tgf-β;Unilateral ureteric obstruction |
Date: | 2017-07 |
Issue Date: | 2018-06-22T04:02:25Z (UTC)
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Publisher: | BMC Nephrol |
ISSN: | 1471-2369 |
Abstract: | BACKGROUND:
The histone deacetylase (HDAC) inhibitor, which has potential effects on epigenetic modifications, had been reported to attenuate renal fibrosis. CD4+ forkhead box P3 (FOXP3)+ T regulatory (Treg) cells may be converted to inflammation-associated T helper 17 cells (Th17) with tissue fibrosis properties. The association between FOXP3+IL-17+ T cells and the attenuation of renal fibrosis by the HDAC inhibitor is not clear.
METHODS:
This study evaluated the roles of the HDAC inhibitor, Treg cells and their differentiation into Th17 cells, which aggravate chronic inflammation and renal fibrosis in a unilateral ureteral obstruction (UUO) mouse model. The study groups included control and UUO mice that were monitored for 7, 14 or 21 days.
RESULTS:
Juxtaglomerular (JG) hyperplasia, angiotensin II type 1 receptor (AT1R) expression and lymphocyte infiltration were observed in renal tissues after UUO but were decreased after trichostatin A (TSA) treatment, a HDAC inhibitor. The number of CD4+FOXP3+ T cells increased progressively, along with the number of FOXP3+interleukin (IL)-17+ T cells, after 14 days, and their numbers then progressively decreased with increasing CD4+IL-17+ T cell numbers, as demonstrated by double immunohistochemistry. Progressive renal fibrosis was associated with the loss of CD4+FOXP3+IL-17+ T cells in splenic single-cell suspensions. FOXP3+IL-17+ T cells expressed TGF-β1 both in vitro and in vivo, and TGF-β1 expression was significantly knockdown by IL-17 siRNA in vitro. These cells were found to play a role in converting Tregs into IL-17- and TGF-β1-producing cells.
CONCLUSIONS:
TSA treatment decreased JG hyperplasia, the percentage of FOXP3+IL-17+ cells and the degree of fibrosis, suggesting that therapeutic benefits may result from epigenetic modifications. |
URI: | http://dx.doi.org/10.1186/s12882-017-0630-6 https://ir.csmu.edu.tw:8080/ir/handle/310902500/19280 |
Relation: | BMC Nephrol. 2017 Jul 10;18(1):225 |
Appears in Collections: | [醫學系] 期刊論文
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