Upregulation of PRMT6 by LPS suppresses Klotho expression through interaction with NF-κB in glomerular mesangial cells

doi:10.1002/jcb.26511

中山醫學大學機構典藏 CSMUIR > 附設醫院 > 期刊論文 > Item 310902500/18985

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题名:	Upregulation of PRMT6 by LPS suppresses Klotho expression through interaction with NF-κB in glomerular mesangial cells
作者:	KD, Tsai WX, Lee Chen, W BY, Chen KL, Chen TC, Hsiao SH, Wang YJ, Lee SY, Liang JC, Shieh TH, Lin
贡献者:	中山醫學大學
关键词:	Klotho;NF-κB;PRMT6;glomerular mesangial cells;lipopolysaccharide
日期:	2018-04-04
上传时间:	2018-03-21T09:21:16Z (UTC)
出版者:	Wiley-Liss Inc.
ISSN:	07302312
摘要:	Lipopolysaccharide (LPS) released from gram-negative bacteria stimulates immune responses in infected cells. Epigenetic modifications such as DNA methylation and protein methylation modulate LPS-induced innate immune gene expressions. Expression of the Klotho protein decreased with LPS treatment in rats. In a cellular model, information regarding the effect of LPS on Klotho expression was meager. In the present study, we demonstrated that LPS triggered global DNA and protein methylation in glomerular mesangial MES-13 cells. LPS upregulated protein expressions of enzymes central to cellular methylation reactions, especially protein arginine methyltransferase 6 (PRMT6) in MES-13 cells. Expression of the Klotho protein was diminished by LPS and was restored by 5-Aza-2'-deoxycytidine (5-Aza-2'-dc), AMI-1, and ammonium pyrrolidinedithiocarbamate (PDTC), but not adenosine aldehyde (AdOx). NF-κB was identified as a substrate for arginine methylation and interacted with PRMT6 in MES-13 cells. Inhibition of PRMT activity by AMI-1 blocked LPS-induced NF-κB nuclear translocation in MES-13 cells. Our data indicate that NF-κB negatively regulated Klotho expression with an interaction with PRMT6, which was upregulated by LPS in MES-13 cells.
URI:	https://www.doi.org/10.1002/jcb.26511 https://ir.csmu.edu.tw:8080/ir/handle/310902500/18985
關聯:	Journal of Cellular Biochemistry Volume 119, Issue 4, April 2018, Pages 3404-3416
显示于类别:	[附設醫院] 期刊論文

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