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    Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18985


    Title: Upregulation of PRMT6 by LPS suppresses Klotho expression through interaction with NF-κB in glomerular mesangial cells
    Authors: KD, Tsai
    WX, Lee
    Chen, W
    BY, Chen
    KL, Chen
    TC, Hsiao
    SH, Wang
    YJ, Lee
    SY, Liang
    JC, Shieh
    TH, Lin
    Contributors: 中山醫學大學
    Keywords: Klotho;NF-κB;PRMT6;glomerular mesangial cells;lipopolysaccharide
    Date: 2018-04-04
    Issue Date: 2018-03-21T09:21:16Z (UTC)
    Publisher: Wiley-Liss Inc.
    ISSN: 07302312
    Abstract: Lipopolysaccharide (LPS) released from gram-negative bacteria stimulates immune responses in infected cells. Epigenetic modifications such as DNA methylation and protein methylation modulate LPS-induced innate immune gene expressions. Expression of the Klotho protein decreased with LPS treatment in rats. In a cellular model, information regarding the effect of LPS on Klotho expression was meager. In the present study, we demonstrated that LPS triggered global DNA and protein methylation in glomerular mesangial MES-13 cells. LPS upregulated protein expressions of enzymes central to cellular methylation reactions, especially protein arginine methyltransferase 6 (PRMT6) in MES-13 cells. Expression of the Klotho protein was diminished by LPS and was restored by 5-Aza-2'-deoxycytidine (5-Aza-2'-dc), AMI-1, and ammonium pyrrolidinedithiocarbamate (PDTC), but not adenosine aldehyde (AdOx). NF-κB was identified as a substrate for arginine methylation and interacted with PRMT6 in MES-13 cells. Inhibition of PRMT activity by AMI-1 blocked LPS-induced NF-κB nuclear translocation in MES-13 cells. Our data indicate that NF-κB negatively regulated Klotho expression with an interaction with PRMT6, which was upregulated by LPS in MES-13 cells.
    URI: https://www.doi.org/10.1002/jcb.26511
    https://ir.csmu.edu.tw:8080/ir/handle/310902500/18985
    Relation: Journal of Cellular Biochemistry Volume 119, Issue 4, April 2018, Pages 3404-3416
    Appears in Collections:[附設醫院] 期刊論文

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