| Abstract: | 青光眼是造成人類致盲之第二大因素,其罹患率逐年上升。其中,急 性高眼壓的傷害因應現代人之生活型態而值得探討。中醫藥古籍中記 載杭菊具有抗發炎、抗氧化、促進血液循環之功效。先前研究顯示杭 菊在乾眼症所引發之角膜損傷與高眼壓引發之視網膜傷害具有緩解作 用,而杭菊對於急性高眼壓所造成的視網膜損傷之緩解功效則是本研 究的重點。本實驗小鼠模式之生理機能與病理分析為主,共分為四組 小鼠:生理對照組、傷害組、低濃度組(100mg/kg)與高濃度組 (500mg/kg),除對照組外皆進境籍性高眼壓傷害;杭菊水萃取物只 餵食低濃度與高濃度組。於實驗第二、九天進行視網膜生理電位測 量;實驗第三、十天進行瞳孔收縮試驗。待實驗結束後犧牲小鼠摘取 眼球進行病理分析。於生理機能上發現:視網膜生理電位之對照組與 高濃度組的電位差皆在0.15mV左右、瞳孔收縮時間皆在2.8秒左右,而 傷害組與低濃度組有則電位差較低、瞳孔收縮時間高於對照組。在病 理分析上,神經節細胞層與視網膜總厚度有隨著餵食濃度增加而增厚 但內核層與外核層無明顯差異。在細胞族群與細胞凋亡部份,視網膜 色素上皮細胞數與神經節細胞數皆隨餵食濃度上升、血管內皮生成因 子、白血球、細胞凋亡訊號隨餵食濃度上升而下降。研究結果顯示餵 食500mg/kg杭菊萃取物能夠有效緩解因急性高眼壓所引發之視網膜傷 害。Glaucoma is the second leading cause of human blindness, with prevalence roaring up year by year. Among many types of glaucoma, acute ocular hypertension leads to damages to retinal ganglion cells and has attracted much attention due to modern life style tend to over-loading eye . According to ancient Chinese herb medicine, Chrysanthemum morifolium flower is regarded as an effective remedy against glaucomatous injuries. Recent studies have confirmed that it has anti-inflammatory and anti- oxidant activities as well as a circulation-promoting effect. Previous studies also demonstrated its ameliorating effects against dry eye induced corneal injuries and ocular hypertension induced retina damages.This study investigated the underlying mechanisms of using Chrysanthemum morifolium flower extract (CMFE) to mitigate the injuries caused by acute ocular hypertension (AOH) on both physiological and pathological aspects. This study based on mouse model including physiological and pathological aspects. The experimental animals randomly divided into 4 groups as control, damage,low dosage(100mg/kg) and high dosage(500mg/ kg).Electroretinogram was performed in day 2,9 and pupil examination was performed in day 3,10. After finish experiment in day 15, the mice were sacrificed and eyes extracted for pathological analyses. In physiological aspects, electroretinogram shows both of blank and high dose in physiological potential difference are around 0.15mV, pupil response time are around 2.8 seconds while damage and low dose group shows lower potential difference and over longer pupil response time. In pathological aspects, retinal ganglion cell layer(GCL) thickness and total retina thickness result shows dose dependence toward blank group while inner nuclear layer(INL) and outer nuclear layer(ONL) shows no significant difference. In cell population and cell apoptosis analysis result, retinal pigment epithelium(RPE) cell number, retinal ganglion cell (RGC) number shows dose dependence toward blank group and vascular endothelial growth factor , white blood cell number and apoptosis signal shows down when CMFE dosage raise. Base on this result, feeding 500mg/kg CMFE prevents retinal ganglia cell and ischemic damages in an AOH glaucoma mouse model. |
