氫氧基磷灰石是人體硬骨組織以及牙齒的主要成分，具有良好的生物相容性及骨傳導性，因此常應用在硬骨再生。先前研究指出鈷離子作為缺氧誘導因子，可加快硬骨組織的再生。本研究以磷酸氫二鈉、硝酸鈣以及氯化亞鈷作為電解液，使用直流式與脈衝式電化學沉積法，在鈦試片上合成鈷取代氫氧基磷灰石，脈衝式電化學沉積法又區分為固定時間與固定電量。結果發現脈衝式電化學沉積法相較於直流式電化學沉積法顯著提高粉體在鈦試片上的產量，生成的鍍層均勻且緻密。感應耦合電漿-質譜分析的結果顯示氫氧基磷灰石中有鈷的存在以及置換率為13.0-14.0％。通過超導量子干涉磁量儀確認本研究所合成的粉體皆具有順磁性，而使用脈衝式電化學沉積法在固定時間下合成的樣品飽和磁化強度高於其他所有樣品。可歸因於該粉體顆粒長寬比接近1，即粒子形狀類似於完美圓形所導致。以小鼠成纖維細胞(L929)和人類骨肉瘤細胞(MG63)進行粉體生物相容性評估，結果證實所合成之鈷取代氫氧基磷灰石具有低毒性。本研究中利用脈衝式電化學沉積法所合成之鈷取代氫氧基磷灰石具有良好的生物活性，具有磁性，可以提高硬骨再生和誘導骨生長的能力，期望未來能進行硬骨修復與核磁共振等方面之應用。Hydroxyapatite (HA) is the main component of human bones and teeth and widely used in bone regeneration based on excellent biocompatibility and bone conduction. In previous study was indicate that cobalt ion as a hypoxia induction factor (HIF-1α) can accelerate bone regeneration. In our study, the electrolyte composition is mixture of sodium dihydrogen phosphate, calcium nitrate and cobalt chloride and the cobalt substituted hydroxyapatite (Co-HA) was synthesized by direct current electrodeposition (DC-CoHA) method and pulsed current electrodeposition (PC-CoHA) method, respectively. Pulse current electrodeposition method in accordance with the current supply mode, divided into fixed time mode (PC-CoHA(fixed time)) and fixed power mode (PC-CoHA(fix power)). The observation shows that PC method fabrication a uniform and dense coating and deposits yield on the titanium significantly higher than DC method. The results of ICP-MS showed that the presence of cobalt on Co-HA and substitution percentage is 13.0 to 14.0%. Co-HA were identified shown to have paramagntic properties by SQUID at 310K. In the other hand, the saturation magnetization of PC-CoHA(fix time) was higher than all the other sample. Attributed to the aspect ratio of the PC-CoHA (fix time) particles near to 1. Therefore, particle shape was similar a perfect sphere. The biocompatibility of Co-HA was evaluated by mouse fibroblasts cells (L929) and human osteosarcoma cells (MG63). The results show that all Co-HA were low toxicity. In our study, PC-CoHA can provide good biological activity, and it has magnetic properties, can improve the ability of bone regeneration and induced bone growth. We hope that the future can be used as hard bone repair and magnetic resonance imaging applications.
