Adverse effects of arecoline and nicotine on human periodontal ligament fibroblasts in vitro

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18399

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题名:	Adverse effects of arecoline and nicotine on human periodontal ligament fibroblasts in vitro
作者:	Chang, Y.-C.;Lii, C.-K.;Tai, K.-W.;Chou, M.-Y.
关键词:	Arecoline/adverse effects;Betel nut;Fibroblasts;Nicotine;Periodontium
日期:	2001
上传时间:	2017-08-14T08:51:04Z (UTC)
ISSN:	0303-6979
摘要:	Background, aims: The habit of betel nut chewing impinges on the daily lives of approximately 200 million people. Betel quid chewers have a higher prevalence of periodontal diseases than non-chewers. This study examined the pathobiological effects of arecoline, a major component of the betel nut alkaloids, on human periodontal ligament fibroblasts (PDLF) in vitro. Method: Cell viability, proliferation, protein synthesis, and cellular thiol levels were used to investigate the effects of human PDLF exposed to arecoline levels of 0 to 200 μg/ml. In addition, nicotine was added to test how it modulated the effects of arecoline. Results: Arecoline significantly inhibited cell proliferation in a dose-dependent manner. At concentrations of 10 and 30 μg/ml, arecoline suppressed the growth of PDLF by 20% and 50% (p<0.05), respectively. Arecoline also decreased protein synthesis in a dose-dependent manner during a 24-h culture period. A 100 μg/ ml concentration level of arecoline significantly inhibited protein synthesis to only 50% of that in the untreated control (p<0.05). Moreover, arecoline significantly depleted intracellular thiols in a dose-dependent manner. At concentrations of 25 μg/ml and 100 μg/ml, arecoline depleted about 18% and 56% of thiols (p<0.05), respectively. This suggests that arecoline itself might augment the destruction of periodontium associated with betel nut use. Furthermore, the addition of nicotine acted with a synergistic effect on the arecoline-induced cytotoxicity. At a concentration of 60 μg/ml, arecoline suppressed the growth of PDLF by about 33%, and 5 mM nicotine enhanced the arecoline-induced cytotoxic response to cause about 66% cell death. Conclusion: During thiol depletion, arecoline may render human PDLF more vulnerable to reactive agents within cigarettes. Taken together, people who combine habits of betel nut chewing with cigarette smoking could be more susceptible to periodontium damage than betel nut chewing alone. Copyright © Munksgaard 2001. All rights reserved.
URI:	http://dx.doi.org/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035289953&partnerID=40&md5=7cce3546f18d48227af169bc1d339f16 https://ir.csmu.edu.tw:8080/ir/handle/310902500/18399
關聯:	Journal of Clinical Periodontology 28 (3) ,277-282
显示于类别:	[牙醫學系暨碩士班] 期刊論文

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