Elevated vimentin expression in buccal mucosal fibroblasts by arecoline in vitro as a possible pathogenesis for oral submucous fibrosis

doi:10.1016/S1368-8375(01)00083-5

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18389

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18389

Title:	Elevated vimentin expression in buccal mucosal fibroblasts by arecoline in vitro as a possible pathogenesis for oral submucous fibrosis
Authors:	Chang, Y.-C.;Tsai, C.-H.;Tai, K.-W.;Yang, S.-H.;Chou, M.-Y.;Lii, C.-K.
Keywords:	Arecoline;Buccal mucosal fibroblasts;Intermediate filament;Oral submucous fibrosis;Vimentin
Date:	2002
Issue Date:	2017-08-14T08:50:23Z (UTC)
ISSN:	1368-8375
Abstract:	Areca quid chewing is strongly correlated with oral submucous fibrosis (OSF) in Taiwan. The cytotoxicity of arecoline, a major areca nut alkaloid, on human oral fibroblasts has been extensively studied. To date, however, there has been little research exploring the possible effects of arecoline on cytoskeleton components. In this study, in addition to conducting a cytotoxicity assay, we examine the effect of arecoline on vimentin, an intermediate filament, and its expression in human buccal mucosal fibroblasts on exposure to various levels of arecoline (0-200 μg/ml) for 48 h. At a concentration above 50 μg/ml, arecoline demonstrated dose-dependent cytotoxicity (P<0.05) for cultured fibroblasts. Using sodium dodecyl sulphate-polyacrylamide gel electrophoresis, we demonstrated dose-dependent elevation of 57 kDa cytoskeletal-protein levels for arecoline. Evidence from immunoblotting assay indicated this 57 kDa cytoskeletal protein was vimentin. The increase in vimentin with arecoline exposure corresponded to that noted for fibroblasts cultured from OSF patients. Immunohistochemical assay also revealed that vimentin expression was much higher for OSF specimens than for normal buccal mucosa. We suggest these results may advance understanding of the possible pathogenesis for submucous fibrosis through the transformation of normal buccal mucosa as a result of areca quid chewing. © 2002 Elsevier Science Ltd. All rights reserved.
URI:	http://dx.doi.org/10.1016/S1368-8375(01)00083-5 https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036317726&doi=10.1016%2fS1368-8375%2801%2900083-5&partnerID=40&md5=55fd0a642cd6e11d4849ab5aa79be81b https://ir.csmu.edu.tw:8080/ir/handle/310902500/18389
Relation:	Oral Oncology 38 (5) ,425-430
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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