Proinflammatory cytokines induce cyclooxygenase-2 mRNA and protein expression in human pulp cell cultures

doi:10.1097/00004770-200303000-00009

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18378

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題名:	Proinflammatory cytokines induce cyclooxygenase-2 mRNA and protein expression in human pulp cell cultures
作者:	Chang, Y.-C.;Yang, S.-F.;Huang, F.-M.;Liu, C.-M.;Tai, K.-W.;Hsieh, Y.-S.
日期:	2003
上傳時間:	2017-08-14T08:49:37Z (UTC)
ISSN:	0099-2399
摘要:	The increased release of prostaglandins (PG) within pulpal tissues is considered to play a pathogenic role during pulpal disease progression. The rate-limiting step in the formation of PG from arachidonic acid is catalyzed by cyclooxygenase (COX). COX-2 is an inducible enzyme believed to be responsible for PG synthesis at site of inflammation. The effect of proinflammatory cytokines on human pulp cells with special reference to COX-2 expression has not been reported earlier. The aim of the present study was to investigate the effects of interleukin (IL)-1α and tumor necrosis factor-α (TNF-α) on the expression of COX-2 mRNA gene and protein in cultured human pulp cells. Investigations of the time dependence of COX-2 mRNA expression in proinflammatory cytokines-treated human pulp cells revealed a rapid accumulation of the transcript, a significant signal first detectable 1 h after exposure. In addition, both IL-1α and TNF-α up-regulated COX-2 protein expression by human pulp cells. The kinetics of this response showed that COX-2 was detectable in cell lysates as early as 2 h post proinflammatory cytokines challenge and remained elevated throughout the 24-h incubation period. This suggests that one of the pathogenic mechanisms of pulpal inflammation in vivo may be the synthesis of COX-2 by resident cells in response to a proinflammatory cytokines challenge. COX-2 may play an important role in the regulation of prostanoid formation in the pathogenesis of pulpal inflammation. Taken together, we propose that the use of selective COX-2 inhibitors might provide a valuable tool in the control of pulpal inflammation. Copyright © 2003 by The American Association of Endodontists.
URI:	http://dx.doi.org/10.1097/00004770-200303000-00009 https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037607471&doi=10.1097%2f00004770-200303000-00009&partnerID=40&md5=3f22450ad434d1a8206c9e99fed1e08f https://ir.csmu.edu.tw:8080/ir/handle/310902500/18378
關聯:	Journal of Endodontics 29 (3) ,201-204
顯示於類別:	[牙醫學系暨碩士班] 期刊論文

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