Induction of cyclooxygenase-2 mRNA and protein expression by epoxy resin and zinc oxide-eugenol based root canal sealers in human osteoblastic cells

doi:10.1016/S0142-9612(02)00584-7

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18373

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18373

Title:	Induction of cyclooxygenase-2 mRNA and protein expression by epoxy resin and zinc oxide-eugenol based root canal sealers in human osteoblastic cells
Authors:	Huang, F.-M.;Chou, M.-Y.;Chang, Y.-C.
Keywords:	Cyclooxygenase-2;Inflammation;Osteoblastic cells;Root canal sealers
Date:	2003
Issue Date:	2017-08-14T08:49:15Z (UTC)
ISSN:	0142-9612
Abstract:	An ideal root canal sealer should be nonirritating to the surrounding tissues. Unfortunately, all histological investigation demonstrated that all types of root canal sealer can induce mild to severe inflammatory alternations. However, there is little information on the precise mechanisms about root canal sealers-induced inflammatory reaction. Cyclooxygenase-2 (COX-2) is an inducible enzyme believed to be responsible for prostaglandin synthesis at site of inflammation. The aim of the present study was to investigate the effects of epoxy resin (AH26) and zinc oxide-eugenol based (Endomethansone and N2) root canal sealers on the expression of COX-2 mRNA gene and protein in cultured human osteoblastic cells. Investigations of the time dependence of COX-2 mRNA expression in root canal sealer-treated human osteoblastic cells revealed a rapid accumulation of the transcript, a significant signal first detectable within 2h and diminished to control level after 24h. In addition, all root canal sealers also induced COX-2 protein expression in human osteoblastic cells. Furthermore, to elucidate whether induction of COX-2 is associated with cytotoxicity, NS-398 (a selective COX-2 inhibitor), was added to test its protective effects. NS-398 at non-cytotoxic dose is not able to prevent root canal sealers-induced cytotoxicity. Taken together, the activation of COX-2 expression may be one of the pathogenesis of root canal sealers-induced periapical inflammation. In addition, root canal sealers-induced cytotoxicity is not directly via the induction of COX-2 expression. © 2003 Elsevier Science Ltd. All rights reserved.
URI:	http://dx.doi.org/10.1016/S0142-9612(02)00584-7 https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037400706&doi=10.1016%2fS0142-9612%2802%2900584-7&partnerID=40&md5=e774fef613b549b933cfd8b006a54406 https://ir.csmu.edu.tw:8080/ir/handle/310902500/18373
Relation:	Biomaterials 24 (11) ,1869-1875
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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