Regulation of interleukin-6 expression by arecoline in human buccal mucosal fibroblasts is related to intracellular glutathione levels

doi:10.1111/j.1601-0825.2004.01041.x

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题名:	Regulation of interleukin-6 expression by arecoline in human buccal mucosal fibroblasts is related to intracellular glutathione levels
作者:	Tsai, C.-H.;Yang, S.-F.;Chen, Y.-J.;Chu, S.-C.;Hsieh, Y.-S.;Chang, Y.-C.
关键词:	Arecoline;Buccal mucosal fibroblasts;Glutathione;Interleukin-6;Oral submucous fibrosis
日期:	2004
上传时间:	2017-08-14T08:48:32Z (UTC)
ISSN:	1354-523X
摘要:	OBJECTIVES: Cytokines play an important role in regulating fibroblast function and is likely to play a key role in regulating the initiation and progression of scarring in any fibrotic disease. Interleukin-6 (IL-6) has been implicated in the development of a variety of fibrotic diseases. The aim of this study was to compare IL-6 expression in fibroblasts cultured from normal human buccal mucosa and oral submucous fibrosis (OSF) specimens and further explore the potential mechanism that may lead to induce IL-6 expression. METHODS: mRNA level of IL-6 in fibroblasts from OSF was compared with normal buccal mucosa. The effects of arecoline, the major areca nut alkaloid, on IL-6 expression in normal human buccal mucosa fibroblasts (BMFs) were measured in vitro. mRNA was quantified with AlphaImager 2000. To determine whether glutathione (GSH) levels were important in the induction of IL-6 by arecoline, we pretreated cells with 2-oxothiazolidine-4-carboxylic acid (OTZ) to boost GSH levels or with buthionine sulfoximine (BSO) to deplete GSH. RESULTS: Fibroblasts derived from OSF exhibited higher IL-6 gene expression than BMF in mRNA levels (P < 0.05). The exposure of quiescent BMF to arecoline resulted in the elevation of IL-6 mRNA expression in a dose-dependent manner (P < 0.05). IL-6 gene regulated by arecoline correlated with intracellular GSH levels in BMF. Arecoline at a concentration of 129 μM induced about 2.7-fold IL-6 mRNA levels over the 6-h incubation period. However, BSO enhanced the IL-6 mRNA levels by 3.9-fold (P < 0.05). In addition, OTZ was found to marginally reduce the arecoline-induced IL-6 expression by about 1.7-fold (P < 0.05). CONCLUSIONS: Taken together, these results suggest that IL-6 expression is significantly upregulated in OSF fibroblasts in areca quid chewers and arecoline may be responsible for the enhanced IL-6 expression. In addition, the regulation of IL-6 expression induced by arecoline is critically dependent on the intracellular GSH concentrations.
URI:	http://dx.doi.org/10.1111/j.1601-0825.2004.01041.x https://www.scopus.com/inward/record.uri?eid=2-s2.0-9444236177&doi=10.1111%2fj.1601-0825.2004.01041.x&partnerID=40&md5=89c37cf23a91bd83b28541b4d3da7017 https://ir.csmu.edu.tw:8080/ir/handle/310902500/18363
關聯:	Oral Diseases 10 (6) ,360-364
显示于类别:	[牙醫學系暨碩士班] 期刊論文

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