Regulation of type i plasminogen activator inhibitor in human gingival fibroblasts with cyclosporine A

doi:10.1111/j.1601-0825.2009.01653.x

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18334

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18334

Title:	Regulation of type i plasminogen activator inhibitor in human gingival fibroblasts with cyclosporine A
Authors:	Ho, Y.-C.;Lin, H.-J.;Tsai, C.-H.;Chang, Y.-C.
Keywords:	Cyclosporine A;Gingival fibroblasts;Gingival overgrowth;Regulatory mechanisms;Type I plasminogen activator inhibitor
Date:	2010
Issue Date:	2017-08-14T08:46:23Z (UTC)
Publisher:	Blackwell Publishing Ltd
ISSN:	1354523X
Abstract:	Oral Diseases (2010) 16, 396-401 Objectives: Cyclosporine A (CsA) is used as an immunosuppressive agent and its prominent side effect is the induction of gingival overgrowth. Type I plasminogen activator inhibitor (PAI-1) has shown to play an important role in CsA-induced gingival overgrowth. However, little is known about whether factors can modulate CsA-induced PAI-1 expression. Methods: Cytotoxicity, reverse transcriptase-polymerase chain reaction, and enzyme-linked immunosorbent assay were used to investigate the effects of Human gingival fibroblasts (HGFs) exposed to CsA. In addition, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, interlukin-1α, tumor necrosis factor-α, mitogen-activated protein kinase kinase (MEK) inhibitor U0126, signal-regulated protein kinase (ERK) inhibitor PD98059 and cell-permeable glutathione precursor N-acetyl-L-cysteine (NAC) were added to test how they modulated the effects of CsA-induced PAI-1 expression. Results: The concentration of CsA higher than 500 ng ml-1 demonstrated cytotoxicity to HGFs (P < 0.05). Periodontal pathogens as well as proinflammatory cytokines were found to increase the CsA-induced PAI-1 mRNA and protein expression (P < 0.05). Pharmacological agents NAC, U0126, and PD98059 were found to decrease the CsA-induced PAI-1 mRNA and protein expression (P < 0.05). Conclusions: Cyclosporine A (CsA) may predispose to gingival overgrowth under inflammatory environments. The regulation of PAI-1 expression induced by CsA might be critically related with the intracellular glutathione and the ERK-MAPK pathway. © 2010 John Wiley & Sons A/S.
URI:	http://dx.doi.org/10.1111/j.1601-0825.2009.01653.x https://www.scopus.com/inward/record.uri?eid=2-s2.0-77950504880&doi=10.1111%2fj.1601-0825.2009.01653.x&partnerID=40&md5=896294e7575058a8e980bfb847c846fd https://ir.csmu.edu.tw:8080/ir/handle/310902500/18334
Relation:	Oral Diseases 16 (4) ,396-401
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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