Platelet-rich fibrin modulates the expression of extracellular signal-regulated protein kinase and osteoprotegerin in human osteoblasts

doi:10.1002/jbm.a.32839

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18329

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18329

Title:	Platelet-rich fibrin modulates the expression of extracellular signal-regulated protein kinase and osteoprotegerin in human osteoblasts
Authors:	Chang, I.-C.;Tsai, C.-H.;Chang, Y.-C.
Keywords:	Choukroun's technique;Osteoblast;Osteoprotegerin;Platelet-rich fibrin;Proliferation
Date:	2010
Issue Date:	2017-08-14T08:46:02Z (UTC)
ISSN:	1549-3296
Abstract:	Platelet-rich fibrin (PRF) by Choukroun's technique is produced in a totally natural manner, without using anticoagulant during blood harvest nor bovine thrombin and calcium chloride for platelet activation and fibrin polymerization. When delicately pressed between two gauzes, the PRF clot becomes a strong membrane with high potential in clinical application and tissue engineering. In this study, blood collection was carried out from healthy volunteers. Osteoblast cell line U2OS was used to evaluate the cell proliferation resulting from PRF by using colorimetric assay. Western blot was employed to evaluate the expression of phosphorylated extracellular signal-regulated protein kinase (p-ERK), receptor activator of nuclear factor-kappa B ligand (RANKL), and osteoprotegerin (OPG) in U2OS cells. PRF was found to increase osteoblast proliferation during 5 day incubation period (p < 0.05). PRF was found to increase ERK phosphorylation in U2OS cells (p < 0.05). OPG was significantly elevated by the stimulation with PRF (p < 0.05). However, there was no significant change in RANKL expression (p > 0.05). Taken together, PRF can stimulate osteoblasts proliferation. The activation of p-ERK and OPG expression by PRF suggests a potential role for new bone formation. The application of PRF may provide the benefit for the bone regeneration. © 2010 Wiley Periodicals, Inc.
URI:	http://dx.doi.org/10.1002/jbm.a.32839 https://www.scopus.com/inward/record.uri?eid=2-s2.0-77956478458&doi=10.1002%2fjbm.a.32839&partnerID=40&md5=41b2d3dfa6392b2d28bf910c511d89e3 https://ir.csmu.edu.tw:8080/ir/handle/310902500/18329
Relation:	Journal of Biomedical Materials Research - Part A 95 (1) ,327-332
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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