Low-level laser effects on simulated orthodontic tension side periodontal ligament cells

doi:10.1089/pho.2012.3359

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18252

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题名:	Low-level laser effects on simulated orthodontic tension side periodontal ligament cells
作者:	Huang, T.-H.;Liu, S.-L.;Chen, Chen C.-L.;Shie, M.-Y.;Kao, C.-T.
日期:	2013
上传时间:	2017-08-09T04:43:25Z (UTC)
ISSN:	15495418
摘要:	Objective: The purpose of this study was to analyze proliferation, inflammation, and osteogenic effects on periodontal ligament (PDL) cells after low-level laser therapy (LLLT) under simulated orthodontic tension conditions. Background data: Low-level lasers affect fibroblast proliferation and collagen synthesis and reduce inflammation. Few studies have focused on the LLLT changes in the PDL caused by moving teeth. Materials and methods: A human PDL cell line was cultured in a -100 kPa tension incubator. The PDL cells were treated with a 670 nm low-level diode laser, output power of 500 mW (continuous wave modus) for 2.5 or 5 sec, spot area 0.25 cm2, corresponding to 1.25 and 2.5 J at an energy density of 5 or 10 J/cm2, respectively. PDL cell viability was assayed by detecting the ability of the cells to cleave tetrazolium salt to formazan dye. Inflammation and osteogenic markers were analyzed by Western blot analysis. Results: PDL cell viablity increased in the experimental group, based on the ability of the cells to cleave tetrazolium salt at day 7 (p<0.05). The experimental group showed no difference in PDL cellular morphology compared with the control group. The inflammation markers inducible NO synthase (iNOS), cyclooxygenase (COX)-2 and interleukin (IL)-1 showed stronger expression in 5 and 10 J/cm2 therapy at days 1 and 5, but decreased in expression at day 7. The osteogenic marker osteocalcin (OC) expression level was significantly higher at day 7 (p<0.05) than in the control cells. Conclusions: LLLT significantly increased PDL cell proliferation, decreased PDL cell inflammation, and increased PDL OC activity under the tension conditions used in this study. © Copyright 2013, Mary Ann Liebert, Inc. 2013.
URI:	http://dx.doi.org/10.1089/pho.2012.3359 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84873361443&doi=10.1089%2fpho.2012.3359&partnerID=40&md5=e14b6bbbceb66c64cf6a056f82ca4883 https://ir.csmu.edu.tw:8080/ir/handle/310902500/18252
關聯:	Photomedicine and Laser Surgery 31(2) ,72-77
显示于类别:	[牙醫學系暨碩士班] 期刊論文

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