Human dental pulp cells responses to apatite precipitation from dicalcium silicates

doi:10.3390/ma8074491

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18228

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18228

Title:	Human dental pulp cells responses to apatite precipitation from dicalcium silicates
Authors:	Lai, W.-Y.;Chen, Y.-W.;Kao, C.-T.;Hsu, T.-T.;Huang, T.-H.;Shie, M.-Y.
Keywords:	Apatite precipitated;Calcium silicate cement;Cell adhesion;Collagen;Fibronectin;Human dental pulp cell
Date:	2015
Issue Date:	2017-08-09T04:42:21Z (UTC)
Publisher:	MDPI AG
ISSN:	19961944
Abstract:	Unraveling the mechanisms behind the processes of cell attachment and the enhanced proliferation that occurs as a response to the presence of calcium silicate-based materials needs to be better understood so as to expand the applications of silicate-based materials. Ions in the environment may influence apatite precipitation and affect silicate ion release from silicate-based materials. Thus, the involvement of apatite precipitate in the regulation of cell behavior of human dental pulp cells (hDPCs) is also investigated in the present study, along with an investigation of the specific role of cell morphology and osteocalcin protein expression cultured on calcium silicate (CS) with different Dulbecco's modified Eagle's medium (DMEM). The microstructure and component of CS cement immersion in DMEM and P-free DMEM are analyzed. In addition, when hDPCs are cultured on CS with two DMEMs, we evaluate fibronectin (FN) and collagen type I (COL) secretion during the cell attachment stage. The facilitation of cell adhesion on CS has been confirmed and observed both by scanning with an electron microscope and using immunofluorescence imaging. The results indicate that CS is completely covered by an apatite layer with tiny spherical shapes on the surface in the DMEM, but not in the P-free DMEM. Compared to the P-free DMEM, the lower Ca ion in the DMEM may be attributed to the formation of the apatite on the surfaces of specimens as a result of consumption of the Ca ion from the DMEM. Similarly, the lower Si ion in the CS-soaked DMEM is attributed to the shielding effect of the apatite layer. The P-free DMEM group releases more Si ion increased COL and FN secretion, which promotes cell attachment more effectively than DMEM. This study provides new and important clues regarding the major effects of Si-induced cell behavior as well as the precipitated apatite-inhibited hDPC behavior on these materials. © 2015 by the authors.
URI:	http://dx.doi.org/10.3390/ma8074491 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84937681700&doi=10.3390%2fma8074491&partnerID=40&md5=496f3e99978d93027de2a17e9bfe0726 https://ir.csmu.edu.tw:8080/ir/handle/310902500/18228
Relation:	Materials 8(7) ,4491-4504
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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