Tensile force on human macrophage cells promotes osteoclastogenesis through receptor activator of nuclear factor κB ligand induction

doi:10.1007/s00774-015-0690-2

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18220

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18220

Title:	Tensile force on human macrophage cells promotes osteoclastogenesis through receptor activator of nuclear factor κB ligand induction
Authors:	Kao, C.-T.;Huang, T.-H.;Fang, H.-Y.;Chen, Y.-W.;Chien, Chien C.-F.;Shie, M.-Y.;Yeh, C.-H.
Keywords:	Human monocyte;Osteoclastogenesis;Receptor activator of nuclear factor κB ligand;Tartrate-resistant acid phosphatase;Tensile force
Date:	2016
Issue Date:	2017-08-09T04:42:00Z (UTC)
Publisher:	Springer-Verlag Tokyo
ISSN:	9148779
Abstract:	Little is known about the effects of tensile forces on osteoclastogenesis by human monocytes in the absence of mechanosensitive cells, including osteoblasts and fibroblasts. In this study we consider the effects of tensile force on osteoclastogenesis in human monocytes. The cells were treated with receptor activator of nuclear factor κB ligand (RANKL) to promote osteoclastogenesis. Then,expression and secretion of cathepsin K were examined. RANKL and the formation of osteoclasts during the osteoclast differentiation process under continual tensile stress were evaluated by Western blot. It was also found that −100 kPa or lower induces RANKL-enhanced tartrate-resistant acid phosphatase activity in a dose-dependent manner. Furthermore, an increased tensile force raises the expression and secretion of cathepsin K elevated by RANKL, and is concurrent with the increase of TNF-receptor-associated factor 6 induction and nuclear factor κB activation. Overall, the current report demonstrates that tensile force reinforces RANKL-induced osteoclastogenesis by retarding osteoclast differentiation. The tensile force is able to modify every cell through dose-dependent in vitro RANKL-mediated osteoclastogenesis, affecting the fusion of preosteoclasts and function of osteoclasts. However, tensile force increased TNF-receptor-associated factor 6 expression. These results are in vitro findings and were obtained under a condition of tensile force. The current results help us to better understand the cellular roles of human macrophage populations in osteoclastogenesis as well as in alveolar bone remodeling when there is tensile stress. © 2015, The Japanese Society for Bone and Mineral Research and Springer Japan.
URI:	http://dx.doi.org/10.1007/s00774-015-0690-2 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84937856820&doi=10.1007%2fs00774-015-0690-2&partnerID=40&md5=9fb5c2f8a5274825fa781811301d8ba2 https://ir.csmu.edu.tw:8080/ir/handle/310902500/18220
Relation:	Journal of Bone and Mineral Metabolism 34(4) ,406-416
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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