本實驗旨在建立一個快速誘導心肌肥大的新動物模型。採完全結紮於兩腎動脈間之腹動脈,導致心肌的血液回流增加,並藉測量在左心室之變化來確認。實驗採多種種系含(SD,WS,LE)大白鼠比較,僅SD大白鼠可以存活。且分析結紮後早期(7天內)血壓,左心室重,全心肌重,及左心室心肌細胞表面積變化。發現除已知的血壓快速上升外,左心室之重量在結紮後3及7天分別比未結紮動物明顯增加28% (P<0.001)及39% (P<0.001)相同於重量的增加,左心室心肌細胞表面積亦分別在手術後3及7天分別增加52% (P<0.05)及92% (P<001)。但在此7天內,並未發現心肌細胞有纖維化的情形。從以上的資料顯示,此SD大白鼠完全腹動脈結紮模式,可作為類週邊血管阻塞、導致心肌肥大及血壓急速上升,造成心肌病變研究最好的動物研究模型。
This report proposes a new model for the study of cardiac hypertrophy. It involves simulated hypertension, i.e., pressure overload, meaning surgically induced through complete coarctation of the abdominal aorta at the junction of renal arteries in rats. The definite advantage of this approach is the speed with which observable results can be measured. A rapid rise in blood pressure was noticed during post-operative intervals in the week following surgery, with significant findings as early as three days post-op. Morphometric analysis of coarcted animals, when compared to sham control specimens, indicated dramatic weight increase in the left ventricle of 28% (P<0.001) at three days following surgery and of 39% (P<0.001) by the seventh day. Upon cross-sectional examination, left ventricular cardiomyocytes showed size expansion of 52% (P<0.05) and 92% (P<0.01) at three and seven days, respectively. Evidence of fibrosis was not detected during the study period. Objectives were completely successful, proving that complete coarctation in rats is a useful model for the study of cardiac pressure overload.