EGCG inhibits transforming growth factor-β-mediated epithelial-to-mesenchymal transition via the inhibition of smad2 and Erk1/2 signaling pathways in nonsmall cell lung cancer cells

doi:10.1021/jf303690x

中山醫學大學機構典藏 CSMUIR > 醫學院 > 醫學系 > 期刊論文 > Item 310902500/18205

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18205

Title:	EGCG inhibits transforming growth factor-β-mediated epithelial-to-mesenchymal transition via the inhibition of smad2 and Erk1/2 signaling pathways in nonsmall cell lung cancer cells
Authors:	Liu, Liu L.-C.;Tsao, Tsao T.C.-Y.;Hsu, S.-R.;Wang, H.-C.;Tsai, Tsai T.-C.;Kao, J.-Y.;Way, T.-D.
Keywords:	EGCG;EMT;nonsmall cell lung cancer;Smad2;TGF-β
Date:	2012
Issue Date:	2017-08-09T03:25:50Z (UTC)
ISSN:	218561
Abstract:	Transforming growth factor-β (TGF-β)-mediated epithelial mesenchymal transition (EMT) of human lung cancer cells may contribute to lung cancer metastasis. It has been reported that EGCG can inhibit tumorigenesis and cancer cell growth in lung cancer; however, the effect of EGCG on EMT in nonsmall cell lung cancer (NSCLC) cells has not been investigated. In this study, we found that NSCLC cells A549 and H1299 were converted to the fibroblastic phenotype in response to TGF-β. Epithelial marker E-cadherin was down-regulated, and mesenchymal marker vimentin was up-regulated simultaneously. Our results illustrated that TGF-β was able to induce EMT in NSCLC cells, and EGCG would reverse TGF-β-induced morphological changes, up-regulate the expression of E-cadherin, and down-regulate the expression of vimentin. Immunofluorescent staining also demonstrated that E-cadherin was up-regulated and that vimentin was down-regulated by EGCG pretreatment. Moreover, wound-healing and the in vitro invasion assay showed that EGCG could inhibit TGF-β-induced migration and invasion of NSCLC cells. By using the dual-luciferase reporter assay, we demonstrated that EGCG inhibited TGF-β-induced EMT at the transcriptional level. EGCG decreased the phosphorylation of Smad2 and Erk1/2, inhibited the nuclear translocation of Smad2, and repressed the expression of transcription factors ZEB1, Snail, Slug, and Twist, and up-regulated the expression of E-cadherin. In summary, our results suggest that EGCG can inhibit TGF-β-induced EMT via down-regulation of phosphorylated Smad2 and Erk1/2 in NSCLC cells. © 2012 American Chemical Society.
URI:	http://dx.doi.org/10.1021/jf303690x https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867045697&doi=10.1021%2fjf303690x&partnerID=40&md5=342994013f9e1f96371a5cae2b159214 https://ir.csmu.edu.tw:8080/ir/handle/310902500/18205
Relation:	Journal of Agricultural and Food Chemistry 60(39) ,9863-9873
Appears in Collections:	[醫學系] 期刊論文

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