Anti-Fenton reaction activity of three taxa of water yam (Dioscorea alata L.)

doi:10.1111/j.1365-2621.2006.01397.x

中山醫學大學機構典藏 CSMUIR > 醫學科技學院 > 生物醫學科學學系暨碩士班 > 期刊論文 > Item 310902500/18058

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Title:	Anti-Fenton reaction activity of three taxa of water yam (Dioscorea alata L.)
Authors:	Wang, T.-S.;Lii, C.-K.;Chang, J.-Y.;Kuo, C.-I.;Chen, H.-P.
Date:	2007
Issue Date:	2017-08-01T08:17:55Z (UTC)
ISSN:	09505423
Abstract:	In the present study, we compared the anti-Fenton reaction activity of three taxa of water yam (Dioscorea alata L.): DS2, TN2 and PSY [D. alata L. var. purpurea (Roxb.) M. Pouch]. Anti-Fenton reaction activity was evaluated by measuring the damage inflicted on calf thymus DNA by copper ions combined with hydrogen peroxide with the use of an ethidium bromide binding assay and agarose gel electrophoresis. We found that extracts of tuber pulp from all three taxa of yam had significant anti-Fenton reaction activity. The protection pattern of the three tuber pulp extracts was similar to that of EDTA, a typical divalent metal ion chelator, which displayed a significant protection lag-phase. With the use of thin-layer chromatography, we found that a common, major ansialdehyde-sulphuric acid stained spot (possibly a polysaccharide mucilage) with an Rf of 0.09 may be the most likely contributor to the anti-Fenton reaction activities of the yam tuber extracts investigated. The present study identifies the mechanism of the health benefit of the Dioscorea family. The copper-chelating and absorbing capability of yam tuber pulp extracts may be useful in functional screening. © 2007 The Authors. Journal compilation 2007 Institute of Food Science and Technology Trust Fund.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/18058 http://dx.doi.org/10.1111/j.1365-2621.2006.01397.x
Relation:	International Journal of Food Science and Technology 42, 1107-1113
Appears in Collections:	[生物醫學科學學系暨碩士班] 期刊論文

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