中山醫學大學機構典藏 CSMUIR:Item 310902500/18056
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    题名: Cigarette smoke extract induces expression of cell adhesion molecules in HUVEC via actin filament reorganization
    作者: Chen, H.-W.;Lii, C.-K.;Ku, H.-J.;Wang, T.-S.
    日期: 2009
    上传时间: 2017-08-01T08:17:51Z (UTC)
    ISSN: 08936692
    摘要: Epidemiologic studies have shown a strong association between cigarette smoking and cardiovascular diseases. Various oxidative species and free radicals are produced during cigarette smoking and these lead to endothelial dysfunction and inflammation. Expression of adhesion molecules, such as intercellular adhesion molecule-1 (ICAM-1), E-selectin, and vascular cell adhesion molecule-1, and adhesion of leukocytes are present in atherosclerosis. We showed previously that a nonfractionated cigarette smoke extract (CSE) induces surface expression of ICAM-1 and E-selectin in human umbilical vein endothelial cells (HUVEC). We then investigated the role of the MAPKs (ERK1/2, JNK, and p38) and AP-1 and the role of actin cytoskeleton reorganization in the CSE-induced expression of ICAM-1 and E-selectin. Western blot analysis showed that CSE treatment rapidly and significantly caused phosphorylation of JNK and ERK1/2 but not of p38. Cytochalasin D (an actin filament disruptor) partially inhibited CSE-induced ICAM-1 and E-selectin surface expression. However, inhibitors of ERK1/2 (PD98059) and JNK (SP600125) did not attenuate the CSE-induced ICAM-1 and E-selectin surface expression. The results of electrophoretic mobility shift assay showed that CSE enhanced AP-1 binding activity. Therefore, CSE activated AP-1 and upregulated ICAM-1 and E-selectin surface expression in HUVEC seem to be via an MAPK-independent pathway. Moreover, the dynamic reorganization of the actin cytoskeleton seems to be required for the CSE-induced surface expression of ICAM-1 and E-selectin. © 2008 Wiley-Liss, Inc.
    URI: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18056
    http://dx.doi.org/10.1002/em.20441
    關聯: Environmental and Molecular Mutagenesis 50, 96-104
    显示于类别:[生物醫學科學學系暨碩士班] 期刊論文

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