Induction of heme oxygenase 1 and inhibition of tumor necrosis factor α-lnduced intercellular adhesion molecule expression by andrographolide in EA.hy926 cells

doi:10.1021/jf101353c

中山醫學大學機構典藏 CSMUIR > 醫學科技學院 > 生物醫學科學學系暨碩士班 > 期刊論文 > Item 310902500/18052

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18052

Title:	Induction of heme oxygenase 1 and inhibition of tumor necrosis factor α-lnduced intercellular adhesion molecule expression by andrographolide in EA.hy926 cells
Authors:	Yu, A.-L.;Lu, C.-Y.;Wang, T.-S.;Tsai, C.-W.;Liu, K.-L.;Cheng, Y.-P.;Chang, H.C.;Lii, C.-K.;Chen, H.-W.
Date:	2010
Issue Date:	2017-08-01T08:17:43Z (UTC)
ISSN:	00218561
Abstract:	Andrographolide is the most abundant diterpene lactone in Andrographis panlculata, which is widely used as a traditional medicine in Southeast Asia. Heme oxygenase 1 (HO-1) is an antioxidant enzyme encoded by a stress-responsive gene. HO-1 has been reported to inhibit the expression of adhesion molecules in vascular endothelial cells (EC). Intercellular adhesion molecule (ICAM-1) is an inflammatory biomarker that is involved in the adhesion of monocytes to EC. In this study, we investigated the effect of andrographolide on the expression of ICAM-1 induced by tumor necrosis factor α (TNF-α) in EA.hy926 cells and the possible mechanisms involved. Andrographolide (2.5-7.5 μM) inhibited the TNF-a-induced expression of ICAM-1 in a dose-dependent manner and resulted in a decrease in HL-60 cell adhesion to EA.hy926 cells (p < 0.05). In parallel, andrographolide significantly induced the expression of HO-1 in a concentration-dependent fashion (p < 0.05). Andrographolide increased the rate of nuclear translocation of nuclear factor erythroid 2-related 2 (Nrf2) and induced antioxidant response element-luciferase reporter activity. Transfection with HO-1-specific small interfering RNA knocked down HO-1 expression, and the inhibition of expression of ICAM-1 by andrographolide was significantly reversed. These results suggest that stimulation of Nrf2-dependent HO-1 expression is involved in the suppression of TNF-a-induced ICAM-1 expression exerted by andrographolide. © 2010 American Chemical Society.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/18052 http://dx.doi.org/10.1021/jf101353c
Relation:	Journal of Agricultural and Food Chemistry 58, 7641-7648
Appears in Collections:	[生物醫學科學學系暨碩士班] 期刊論文

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