Anticlastogenic effect of aqueous extract from water yam (Dioscorea alata L.)

doi:10.5897/JMPR11.1126

中山醫學大學機構典藏 CSMUIR > 醫學科技學院 > 生物醫學科學學系暨碩士班 > 期刊論文 > Item 310902500/18048

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18048

Title:	Anticlastogenic effect of aqueous extract from water yam (Dioscorea alata L.)
Authors:	Wang, T.-S.;Lii, C.-K.;Huang, Y.-C.;Chang, J.-Y.;Yang, F.-Y.
Date:	2011
Issue Date:	2017-08-01T08:17:34Z (UTC)
ISSN:	19960875
Abstract:	Dioscorea plants have been widely used as traditional medicine and food for health benefits. Several therapeutic properties such as anti-cough, anti-diabetic, anti-diarrhea, and anti-cancer have been reported. Although the steroidal saponins (such as diosgenin) account for some of Dioscorea's activity, there is little information on the effective components. We have shown here that water yam (Dioscorea alata L.) aqueous extract (YAE) inhibited the H2O2-CuSO4 induced damage of calf thymus DNA, and protected cultured human lymphoblastoid cells from CuSO4-induced DNA damage. This aqueous extract exhibited a major absorption peak at 260 to 262 nm. Upon the addition of CuSO4, the absorption peak was shifted to 236 nm and it was sensitive to the divalent ion chelator EDTA. The aqueous extract of YAE was also capable of dissociating the complex formation of Cu (II) with tetramethyl murexide (a copper indicator). Thin layer chromatography indicated that it contains one major and several minor anisaldehyde-, sulfuric acid-reactive and highly polar compounds. Based on these findings, we suggest that water-soluble mucilaginous polysaccharides within YAE are likely the effective compounds/candidates, which carry copper-chelating activity, and protect DNA against H2O2-CuSO4 combination or CuSO4 alone. © 2011 Academic Journals.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/18048 http://dx.doi.org/10.5897/JMPR11.1126
Relation:	Journal of Medicinal Plant Research 5, 6192-6202
Appears in Collections:	[生物醫學科學學系暨碩士班] 期刊論文

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