Metastasis tumor-associated protein-2 knockdown suppresses the proliferation and invasion of human glioma cells in vitro and in vivo

doi:10.1007/s11060-014-1558-3

中山醫學大學機構典藏 CSMUIR > 醫學院 > 醫學系 > 期刊論文 > Item 310902500/17962

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题名:	Metastasis tumor-associated protein-2 knockdown suppresses the proliferation and invasion of human glioma cells in vitro and in vivo
作者:	Cheng, Chun-Yuan Chou, Ying-Erh Ko, Chung-Po Yang, Shun-Fa Hsieh, Shu-Ching Lin, Chia-Liang Hsieh, Yi-Hsien Chen, Kun-Chung
贡献者:	中山醫大
关键词:	MTA2;Glioma;Proliferation;Migration;Invasion;Tumorigenicity;Immunohistochemistry
日期:	2014
上传时间:	2017-07-11T09:06:55Z (UTC)
出版者:	Journal of Neuro-Oncology
ISSN:	0167-594X
摘要:	Abstract Metastasis tumor-associated protein 2 (MTA2) is a member of the MTA family that is closely associated with tumor progression and metastasis. However, the role of MTA2 in glioma cells remains unclear. The expression of MTA2 was measured using immunohistochemistry and western blotting in the human brain tumor tissue array and human glioma cell lines. The impact of MTA2 knockdown on GBM8401 and Hs683 cell growth was evaluated by MTT assay and flow cytometry. Cell migration and invasion were analyzed by cell-migration assay and Matrigel invasion assay. In addition, we used subcutaneous tumor models to study the effect of MTA2 on the growth of glioma cells in vivo. We found that MTA2 protein and mRNA expression are higher in GBM8401 and Hs683 cells than in other glioma cells (M059 J, M059 K and U-87 MG), and glioma tumor tissue correlated significantly with tumor grade (P < 0.001). Knockdown of MTA2 expression significantly inhibited cell growth, cell migration and invasion, and induced G0/G1 phase arrest in human GBM8401 and Hs683 cells in vitro. Moreover, in vivo studies using subcutaneous xenografts in mice models indicate that MTA2 knockdown significantly inhibited tumorigenicity. These results indicate that MTA2 plays an important oncogenic role in the development and progression of gliomas.
URI:	http://dx.doi.org/10.1007/s11060-014-1558-3 https://ir.csmu.edu.tw:8080/ir/handle/310902500/17962
關聯:	Journal of Neuro-Oncology November 2014, Volume 120, Issue 2, pp 273–281
显示于类别:	[醫學系] 期刊論文

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