Danshen mediates through estrogen receptors to activate Akt and inhibit apoptosis effect of Leu27IGF-II-induced IGF-II receptor signaling activation in cardiomyoblasts

doi:10.1016/j.fct.2013.01.008

中山醫學大學機構典藏 CSMUIR > 醫學科技學院 > 醫學檢驗暨生物技術學系暨碩士班 > 期刊論文 > Item 310902500/17910

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题名:	Danshen mediates through estrogen receptors to activate Akt and inhibit apoptosis effect of Leu27IGF-II-induced IGF-II receptor signaling activation in cardiomyoblasts
作者:	YS, Weng WW, Kuo YM, Lin CH, Kuo BS, Tzang FJ, Tsai CH, Tsai JA, Lin DJ, Hsieh CY, Huang
贡献者:	中山醫大
关键词:	Danshen (Salvia miltiorrhiza);Estrogen receptors;IGF-II receptor;Cardiac apoptosis;p-Akt
日期:	2013
上传时间:	2017-07-04T08:32:22Z (UTC)
出版者:	Food Chem Toxicol
ISSN:	0278-6915
摘要:	Abstract Post-menopausal women show dramatically increased cardiovascular disease morbidity (CVD). Danshen is used widely in China for the treatment of cardiovascular disorders, including coronary heart disease. Danshen possesses lipid-soluble biologically active components with a structure similar to 17β-estrodiol (E2). This study assesses whether the cardio-protection exerted by Danshen is mediated through the ERs within H9c2 cardiomyoblast cells. Cardiomyoblast cells pretreated with Fulvestrant (ICI 182,780), an estrogen receptor antagonist was applied to investigate the estrogenic activity of Danshen. The Danshen extract preventive effects on Leu27IGF-II-induced IGF-IIR signaling activator and H9c2 cell apoptosis were identified using TUNEL assay, JC-1 staining and Western blot assay. We found that Danshen extract treatments significantly enhanced phosphorylated Akt through estrogen receptor activation to inhibit Leu27IGF-II-induced calcineurin activation and block H9c2 cell apoptosis. Danshen extracts suppressed the IGF-IIR signaling proteins, pro-apoptotic proteins and reversed the mitochondrial membrane instability induced by Leu27IGF-II. However, the cardioprotective properties of Danshen to inhibit Leu27IGF-II-induced cell apoptosis and promote cell survival were attenuated by applying ICI, which suggests that the Danshen cardioprotective effect is mediated through estrogen receptors. All our data indicated that Danshen exerts strong estrogenic activity which can be considered a novel selective estrogen receptor modulator (SERM) against IGF2R signaling that blocks cardiac apoptosis.
URI:	http://dx.doi.org/10.1016/j.fct.2013.01.008 https://ir.csmu.edu.tw:8080/ir/handle/310902500/17910
關聯:	Food Chem Toxicol. 2013 Jun;56:28-39.
显示于类别:	[醫學檢驗暨生物技術學系暨碩士班] 期刊論文

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