Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens

doi:10.1016/S1413-8670(12)70318-5

中山醫學大學機構典藏 CSMUIR > 醫學院 > 醫學系 > 期刊論文 > Item 310902500/17878

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/17878

Title:	Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens
Authors:	HC, Hung CH, Chan SM, Tsao SC, Chen TC, Wu MC, Lu DJ, Wu SJ, Huang YT, Lee
Contributors:	中山醫學大學
Keywords:	Bronchoalveolar lavage;Mycobacterium tuberculosis;Acid-fast bacilli staining;Molecular diagnosis;BDProbeTEC ET system
Date:	2012
Issue Date:	2017-06-29T09:32:06Z (UTC)
Publisher:	The Brazilian Journal of Infectious Diseases
ISSN:	1413-8670
Abstract:	Abstract Objective The diagnostic efficacy of the BDProbeTEC ET Mycobacterium tuberculosis (MTB) complex direct detection assay (DTB) performed on bronchoalveolar lavage (BAL) specimens and sputum smears was compared with acid-fast bacilli (AFB) smear microscopy. Method AFB smear microscopy, DTB and culture results of 286 patients with pulmonary tuberculosis were retrospectively reviewed. A total of 120 patients provided expectorated sputum samples, and 166 patients provided BAL specimens. Culture results and clinical diagnosis were used as gold standards. Results The sensitivity and specificity of the DTB assay in detecting MTB in sputum specimens was significantly higher compared to AFB smear microscopy (83.7% and 82.4%, vs. 75.6%, and 41.2%, respectively). The sensitivity and specificity of the DTB assay in detecting MTB in sputum samples was 77.2% and 100% compared to clinical diagnosis, while AFB smear had a sensitivity and specificity of 70.3% and 26.3%, respectively. Compared to culture, DTB had a sensitivity and specificity of 82.8% and 93.2%, respectively, in detecting MTB from BAL specimens; AFB smear had a sensitivity and specificity of 41.9% and 87.7%, respectively. Compared to clinical diagnosis, DTB had a sensitivity and specificity of 67.2% and 100%, respectively, in detecting MTB from BAL specimens; AFB smear had a sensitivity and specificity of 34.8% and 79.5%, respectively. Conclusions The superior performance of the DTB assay relative to AFB smear microscopy makes it a valuable tool to enable early diagnosis of MTB, thereby improving patient care and reducing transmission.
URI:	https://doi.org/10.1016/S1413-8670(12)70318-5 https://ir.csmu.edu.tw:8080/ir/handle/310902500/17878
Relation:	The Brazilian Journal of Infectious Diseases Volume 16, Issue 3, May–June 2012, Pages 242-249
Appears in Collections:	[醫學系] 期刊論文

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