In vitro and in vivo targeted delivery of IL-10 interfering RNA by JC virus-like particles

doi:10.1186/1423-0127-17-51

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/17875

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题名:	In vitro and in vivo targeted delivery of IL-10 interfering RNA by JC virus-like particles
作者:	Chou, Meng-Ing Hsieh, Yu-Fan Wang, Meilin Chang, Jinghua Tsai Chan, Deching Zouali, Moncef Tsay, Gregory J
贡献者:	中山醫學大學
日期:	2010
上传时间:	2017-06-29T09:15:45Z (UTC)
ISSN:	1021-7770
摘要:	Abstract Background RNA interference (RNAi) is a powerful tool to silence gene expression post-transcriptionally. Delivering sequences of RNAi in vivo remains a problem. The aim of this study was to use JC virus (JCV) virus-like particles (VLPs) as a vector for delivering RNAi in silencing the cytokine gene of IL-10. Methods JCV VLPs were generated by recombinant JCV VP1 protein in yeast expression system. DNA fragment containing IL-10 shRNA was packaged into VLPs by osmotic shock. Results In RAW 264.7 cells, IL-10 shRNA was found to reduce IL-10 expression by 85 to 89%, as compared with VLPs alone. IL-10 shRNA did not cross-react with TNF-alpha mRNA or influence the expression of TNF-alpha. In BALB/c mice IL-10 shRNA could reduce 95% of IL-10 secretion. Surprisingly, it also down regulated TNF-alpha expression. Conclusions We show for the first time that JCV VLPs empty capsids are competent vectors to deliver RNAi and are nontoxic to cells, suggesting that JCV VLPs is an efficient agent to deliver RNAi in both murine macrophage cells and BALB/c mice. This system provides an efficient means for delivering the RNAi for gene therapy purposes.
URI:	http://dx.doi.org/10.1186/1423-0127-17-51 https://ir.csmu.edu.tw:8080/ir/handle/310902500/17875
關聯:	Journal of Biomedical Science
显示于类别:	[牙醫學系暨碩士班] 期刊論文

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