中山醫學大學機構典藏 CSMUIR:Item 310902500/17842
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    题名: The upregulation of transglutaminase-2 by cyclosporin a in human gingival fibroblasts is augmented by oxidative stress
    作者: Lee, Shiuan-Shinn
    Tsai, Chung-Hung
    Kuan, Yu-Hsiang
    Huang, Fu-Mei
    Chang, Yu-Chao
    贡献者: 中山醫大
    日期: 2012
    上传时间: 2017-06-28T08:14:25Z (UTC)
    出版者: Journal of Periodontology
    ISSN: 0022-3492
    摘要: Abstract
    BACKGROUND:
    Transglutaminase-2 (TGM-2) has been implicated in several fibrotic disorders and can be induced by reactive oxygen species (ROS). Hence, the authors hypothesize that cyclosporin A (CsA) may regulate TGM-2 via ROS, and this regulation may have a role in the pathogenesis of CsA-induced gingival overgrowth.
    METHODS:
    Cytotoxicity, 2',7'-dichlorodihydrofluorescein diacetate assay, and Western blot were used to investigate the effects of CsA in human gingival fibroblasts (HGFs). In addition, extracellular signal-regulated kinase (ERK) inhibitor PD98059, phosphatidylinositol 3-kinase inhibitor LY294002, glutathione precursor N-acetyl-L-cysteine (NAC), curcumin, epigallocatechin-3 gallate (EGCG), and p38 inhibitor SB203580 were added to find the possible regulatory mechanisms.
    RESULTS:
    Concentrations of CsA >500 ng/mL demonstrated cytotoxicity to HGFs (P < 0.05). CsA enhanced the generation of intracellular ROS at concentrations >200 ng/mL (P <0.05). TGM-2 protein induced by CsA was found in HGFs in a dose- and time-dependent manner (P <0.05). The addition of PD98059, LY294002, NAC, curcumin, EGCG, and SB203580 markedly inhibited TGM-2 expression induced by CsA (P <0.05).
    CONCLUSIONS:
    These results demonstrate that CsA significantly upregulates intracellular ROS generation and
    URI: http://dx.doi.org/10.1902/jop.2012.120554
    https://ir.csmu.edu.tw:8080/ir/handle/310902500/17842
    關聯: Journal of Periodontology October 2013, Vol. 84, No. 10, Pages 1469-1475
    显示于类别:[公共衛生學系暨碩士班] 期刊論文

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